概述

  • 产品名称Anti-ERAP2抗体
    参阅全部 ERAP2 一抗
  • 描述
    山羊多克隆抗体to ERAP2
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Cow, Orangutan
  • 免疫原

    Peptide with sequence C-

    QYLKKFSYRNAKND

    , corresponding to internal sequence amino acids 480-493 of human ERAP2 (NP_071745.1).

  • 阳性对照
    • Human spleen lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Purified from goat serum by ammonium sulphate precipitation, followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab82142 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
  • 应用说明Peptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1:16,000.
    WB: Use at a concentration of 0.5 - 1.5 µg/ml. Predicted molecular weight: 110 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性ERAP2 is an aminopeptidase that plays a central role in peptide trimming. Major histocompatibility complex (MHC) class I molecules rely on aminopeptidases such as ERAP2 to trim precursors to antigenic peptides in the endoplasmic reticulum (ER) following cleavage in the cytoplasm by tripeptidyl peptidase II. ERAP2 preferentially hydrolyzes the basic residues Arg and Lys.
    • 细胞定位Endoplasmic reticulum membrane; Single-pass type II membrane protein.
    • 数据库链接
    • 别名
      • Endoplasmic reticulum aminopeptidase 2 antibody
      • L RAP antibody
      • Leukocyte derived arginine aminopeptidase antibody
      • LRAP antibody

    Anti-ERAP2 antibody 图像

    • Anti-ERAP2 antibody (ab82142) at 0.5 µg/ml + human spleen lysate in RIPA buffer at 35 µg
      Developed using the ECL technique

      Predicted band size : 110 kDa
      Observed band size : 110 kDa
      Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 1 hour

    Anti-ERAP2 antibody (ab82142)参考文献

    ab82142 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"