概述

  • 产品名称Anti-ERAP2抗体
    参阅全部 ERAP2 一抗
  • 描述
    小鼠多克隆抗体to ERAP2
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length human ERAP2 protein (AAH17927, 350 a.a)

  • 阳性对照
    • Human kidney tissue lysate. ERAP2 transfected 293T cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab69037 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明WB: 1/500 - 1/1000. Detects bands of approximately 110 and 40 kDa (predicted molecular weight: 110 kDa; Isoform 4, 40 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性ERAP2 is an aminopeptidase that plays a central role in peptide trimming. Major histocompatibility complex (MHC) class I molecules rely on aminopeptidases such as ERAP2 to trim precursors to antigenic peptides in the endoplasmic reticulum (ER) following cleavage in the cytoplasm by tripeptidyl peptidase II. ERAP2 preferentially hydrolyzes the basic residues Arg and Lys.
    • 细胞定位Endoplasmic reticulum membrane; Single-pass type II membrane protein.
    • 数据库链接
    • 别名
      • Endoplasmic reticulum aminopeptidase 2 antibody
      • L RAP antibody
      • Leukocyte derived arginine aminopeptidase antibody
      • LRAP antibody

    Anti-ERAP2 antibody 图像

    • Anti-ERAP2 antibody (ab69037) at 1/500 dilution + Human kidney lysate at 50 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 110 kDa
      Observed band size : 110 kDa
    • All lanes : Anti-ERAP2 antibody (ab69037) at 1/500 dilution

      Lane 1 : ERAP2 transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 110 kDa
      Observed band size : 40 kDa (why is the actual band size different from the predicted?)

    Anti-ERAP2 antibody (ab69037)参考文献

    This product has been referenced in:
    • Kuiper JJ  et al. A genome-wide association study identifies a functional ERAP2 haplotype associated with birdshot chorioretinopathy. Hum Mol Genet 23:6081-7 (2014). Human . Read more (PubMed: 24957906) »
    • Wang B  et al. p53 increases MHC class I expression by upregulating the endoplasmic reticulum aminopeptidase ERAP1. Nat Commun 4:2359 (2013). WB . Read more (PubMed: 23965983) »

    See all 3 Publications for this product

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