概述

  • 产品名称Anti-ERAB抗体
    参阅全部 ERAB 一抗
  • 描述
    兔多克隆抗体to ERAB
  • 特异性ab52243 detects endogenous levels of total ERAB protein.
  • 经测试应用适用于: ICC/IF, WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide derived from human ERAB.

  • 阳性对照
    • LOVO cells. HeLa cell line

性能

应用

Our Abpromise guarantee covers the use of ab52243 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
WB 1/300 - 1/1000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).
ELISA 1/10000.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能Functions in mitochondrial tRNA maturation. Part of mitochondrial ribonuclease P, an enzyme composed of MRPP1/RG9MTD1, MRPP2/HSD17B10 and MRPP3/KIAA0391, which cleaves tRNA molecules in their 5'-ends. By interacting with intracellular amyloid-beta, it may contribute to the neuronal dysfunction associated with Alzheimer disease (AD).
  • 组织特异性Expressed in normal tissues but is overexpressed in neurons affected in AD.
  • 疾病相关Defects in HSD17B10 are the cause of 2-methyl-3-hydroxybutyryl-CoA dehydrogenase deficiency (MHBD deficiency) [MIM:300438]. MHBD deficiency leads to neurological abnormalities, including psychomotor retardation, and, in virtually all patients, loss of mental and motor skills.
    Defects in HSD17B10 are the cause of mental retardation syndromic X-linked type 10 (MRXS10) [MIM:300220]. MRXS10 is characterized by mild mental retardation, choreoathetosis and abnormal behavior.
    A chromosomal microduplication involving HSD17B10 and HUWE1 is the cause of mental retardation X-linked type 17 (MRX17) [MIM:300705]; also known as mental retardation X-linked type 31 (MRX31). Mental retardation is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. In contrast to syndromic or specific X-linked mental retardation which also present with associated physical, neurological and/or psychiatric manifestations, intellectual deficiency is the only primary symptom of non-syndromic X-linked mental retardation.
  • 序列相似性Belongs to the short-chain dehydrogenases/reductases (SDR) family.
  • 细胞定位Mitochondrion.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 17 beta hydroxysteroid dehydrogenase 10 antibody
    • 17 beta hydroxysteroid dehydrogenase type 10 antibody
    • 17-beta-HSD 10 antibody
    • 17-beta-hydroxysteroid dehydrogenase 10 antibody
    • 17b HSD10 antibody
    • 3 hydroxy 2 methylbutyryl CoA dehydrogenase antibody
    • 3 hydroxyacyl CoA dehydrogenase type 2 antibody
    • 3 hydroxyacyl CoA dehydrogenase type II antibody
    • 3-hydroxy-2-methylbutyryl-CoA dehydrogenase antibody
    • 3-hydroxyacyl-CoA dehydrogenase type II antibody
    • 3-hydroxyacyl-CoA dehydrogenase type-2 antibody
    • AB binding alcohol dehydrogenase antibody
    • ABAD antibody
    • Ads9 antibody
    • Amyloid beta binding polypeptide antibody
    • Amyloid beta peptide binding alcohol dehydrogenase antibody
    • Amyloid beta peptide binding protein antibody
    • Amyloid beta peptide binding protein antibody
    • CAMR antibody
    • DUPXp11.22 antibody
    • Endoplasmic Reticulum Amyloid Binding Protein antibody
    • Endoplasmic reticulum associated amyloid beta peptide binding protein antibody
    • Endoplasmic reticulum-associated amyloid beta-peptide-binding protein antibody
    • ER associated amyloid beta-binding protein antibody
    • ERAB antibody
    • HADH 2 antibody
    • HADH2 antibody
    • HCD 2 antibody
    • HCD2 antibody
    • HCD2_HUMAN antibody
    • Hsd17b10 antibody
    • Hydroxyacyl CoA Dehydrogenase type II antibody
    • Hydroxyacyl Coenzyme A dehydrogenase type II antibody
    • Hydroxysteroid (17 beta) dehydrogenase 10 antibody
    • Mental retardation X linked syndromic 11 antibody
    • MHBD antibody
    • Mitochondrial L3 Hydroxyacyl CoA Dehydrogenase antibody
    • Mitochondrial ribonuclease P protein 2 antibody
    • Mitochondrial RNase P protein 2 antibody
    • MRPP2 antibody
    • MRX17 antibody
    • SCHAD antibody
    • SDR5C1 antibody
    • Short chain dehydrogenase/reductase family 5C member 1 antibody
    • Short chain L 3 hydroxyacyl CoA dehydrogenase type 2 antibody
    • Short chain type dehydrogenase/reductase XH98G2 antibody
    • Short-chain type dehydrogenase/reductase XH98G2 antibody
    • Type 10 17b HSD antibody
    • Type 10 17beta hydroxysteroid dehydrogenase antibody
    • Type II HADH antibody
    • XH98G2 antibody
    see all

Anti-ERAB antibody 图像

  • All lanes : Anti-ERAB antibody (ab52243) at 1/300 dilution

    Lane 1 : LOVO cell extract; without peptide
    Lane 2 : LOVO cell extract; with peptide


    Predicted band size : 27 kDa
    Observed band size : 27 kDa
  • Ab52243 staining human normal lung tissue. Staining is localised to mitochondria.
    Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • ICC/IF image of ab55243 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52243, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-ERAB antibody (ab52243)参考文献

This product has been referenced in:
  • Yao J  et al. Ovarian hormone loss induces bioenergetic deficits and mitochondrial ß-amyloid. Neurobiol Aging : (2011). WB ; Mouse . Read more (PubMed: 21514693) »
  • Yao J  et al. Mitochondrial bioenergetic deficit precedes Alzheimer's pathology in female mouse model of Alzheimer's disease. Proc Natl Acad Sci U S A 106:14670-5 (2009). WB ; Mouse . Read more (PubMed: 19667196) »

See all 2 Publications for this product

Product Wall

Thank you for contacting us and your interest in our products. I am pleased to provide the following immunogen information: xxxxxx. In order to aid your research we are happy to provide this information to you. However, this immunogen sequence ...

Read More

Thank you for your inquiry. The immunogen sequence is within the range of a.a. 100 to a.a. 200 of human ERAB.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Mouse brain hippocampi lysate)
Loading amount 20 µg
Specification Mouse brain hippocampi lysate
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Aug 06 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"