RabMAb

Anti-Epsin 1抗体[EPR3023] (ab75879)

概述

  • 产品名称Anti-Epsin 1抗体[EPR3023]
    参阅全部 Epsin 1 一抗
  • 描述
    兔单克隆抗体[EPR3023] to Epsin 1
  • 经测试应用适用于: WB, ICC/IFmore details
    不适用于: IHC-P
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Epsin 1 aa 450-550.
    Database link: Q9Y6I3

  • 阳性对照
    • ICC/IF: SH-SY5Y cells WB: SH-SY5Y and HeLa cell lysates
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab75879 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/2000. Predicted molecular weight: 58 kDa.
ICC/IF 1/50.

For unpurified, use 1/100 - 1/250.

  • 应用说明Is unsuitable for IHC-P.
  • 靶标

    • 功能Binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). Modifies membrane curvature and facilitates the formation of clathrin-coated invaginations (By similarity). Regulates receptor-mediated endocytosis.
    • 序列相似性Belongs to the epsin family.
      Contains 1 ENTH (epsin N-terminal homology) domain.
      Contains 3 UIM (ubiquitin-interacting motif) repeats.
    • 结构域The NPF repeat domain is involved in EPS15 binding.
      The DPW repeat domain is involved in AP2A2 and clathrin binding.
      The [DE]-X(1,2)-F-X-X-[FL]-X-X-X-R motif mediates interaction with the AP-2 complex subunit AP2B1.
    • 翻译后修饰Phosphorylated on serine and/or threonine residues in mitotic cells. Phosphorylation reduces interaction with REPS2, AP-2 and the membrane fraction. Depolarization of synaptosomes results in dephosphorylation.
      Ubiquitinated.
    • 细胞定位Cytoplasm. Cell membrane. Nucleus. Membrane > clathrin-coated pit. Associated with the cytoplasmic membrane at sites where clathrin-coated pits are forming. Colocalizes with clathrin and AP-2 in a punctate pattern on the plasma membrane. Detected in presynaptic nerve terminals and in Golgi stacks. May shuttle to the nucleus when associated with ZBTB16/ZNF145.
    • Information by UniProt
    • 数据库链接
    • 别名
      • EH domain binding mitotic phosphoprotein antibody
      • EH domain-binding mitotic phosphoprotein antibody
      • EPN 1 antibody
      • Epn1 antibody
      • EPN1_HUMAN antibody
      • EPS 15 interacting protein 1 antibody
      • EPS-15-interacting protein 1 antibody
      • Epsin 1 antibody
      • Epsin-1 antibody
      see all

    Anti-Epsin 1 antibody [EPR3023] 图像

    • Anti-Epsin 1 antibody [EPR3023] (ab75879) at 1/5000 dilution (purified) + rat brain at 20 µg

      Secondary
      HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

      Predicted band size : 58 kDa
      Observed band size : 94 kDa (why is the actual band size different from the predicted?)

      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-Epsin 1 antibody [EPR3023] (ab75879) at 1/1000 dilution (purified)

      Lane 1 : 293T cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : U87-MG cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP goat anti-rabbit IgG (H+L) at 1/10000 dilution

      Predicted band size : 58 kDa
      Observed band size : 94 kDa (why is the actual band size different from the predicted?)

      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Anti-Epsin 1 antibody [EPR3023] (ab75879) at 1/5000 dilution (purified) + mouse brain lysate at 20 µg

      Secondary
      HRP goat anti-rabbit IgG (H+L) at 1/10000 dilution

      Predicted band size : 58 kDa
      Observed band size : 94 kDa (why is the actual band size different from the predicted?)

      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Immunofluorescence staining of HeLa cells with purified ab75879 at a working dilution of 1/50, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab75879 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

    • Unpurified ab75879, at a 1/100 dilution, staining Epsin 1 in SH-SY5Y cells by Immunofluorescence.

    • All lanes : Anti-Epsin 1 antibody [EPR3023] (ab75879) at 1/2000 dilution (unpurified)

      Lane 1 : SH-SY5Y cell lysate
      Lane 2 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit-HRP at 1/1000 dilution
      Developed using the ECL technique

      Predicted band size : 58 kDa

    Anti-Epsin 1 antibody [EPR3023] (ab75879)参考文献

    ab75879 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting us. I have quickly checked and found that this protein indeed runs at 80-90 kDa. The 58 kDa is the molecular weight of unprocessed raw protein which is calculated by combining the weight of amino acids as advised in Swisspr...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (astrocytoma)
    Loading amount 25 µg
    Specification astrocytoma
    Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
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    提交于 Apr 09 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"