A region between residue 600 and 650 of human EPS8L2 (NP_073609.2)
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Preservative: 0.09% Sodium Azide
Constituents: Tris citrate/phosphate, pH 7-8
Concentration information loading...
Immunogen affinity purified
ab85960 was affinity purified using an epitope specific to EPS8L2 immobilized on solid support.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Detects a band of approximately 81 kDa (predicted molecular weight: 81 kDa).
Use at 2-5 µg/mg of lysate.
Stimulates guanine exchange activity of SOS1. May play a role in membrane ruffling and remodeling of the actin cytoskeleton.
Detected in fibroblasts and placenta.
Belongs to the EPS8 family.
Contains 1 PID domain. Contains 1 SH3 domain.
Information by UniProt
Epidermal growth factor receptor kinase substrate 8 like protein 2 antibody
Epidermal growth factor receptor kinase substrate 8-like protein 2 antibody
Epidermal growth factor receptor pathway substrate 8 like protein 2 antibody
Western blot - Anti-EPS8L2 antibody (ab85960)
All lanes : Anti-EPS8L2 antibody (ab85960) at 0.4 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Developed using the ECL technique. Predicted band size: 81 kDa Observed band size: 81 kDa Exposure time: 30 seconds
Immunoprecipitation - Anti-EPS8L2 antibody (ab85960)
Detection of Human EPS8L2 by Immunoprecipitation, using ab85960 at 3µg/mg lysate. Image shows immunoprecipitated EPS8L2 detected post IP by WB, loading 20% of IP using HeLa whole cell lysate at 1mg, with ab85960 at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
has not yet been referenced specifically in any publications.
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