重组Anti-EPS8抗体[EPR6112] (ab124882)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6112] to EPS8
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-EPS8抗体[EPR6112]
参阅全部 EPS8 一抗 -
描述
兔单克隆抗体[EPR6112] to EPS8 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human EPS8 aa 1-100 (N terminal). The exact sequence is proprietary.
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阳性对照
- HeLa, A375, 293T, and K562 cell lysates, Human kidney tissue
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR6112 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124882于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000 - 1/10000. Detects a band of approximately 97 kDa (predicted molecular weight: 92 kDa).
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
antigen retrieval is recommended. |
说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 97 kDa (predicted molecular weight: 92 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. antigen retrieval is recommended. |
靶标
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功能
Upon binding to EGF receptor enhances EGF-dependent mitogenic signals. Can bind multiple cellular targets. -
组织特异性
Expressed in all tissues analyzed, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Expressed in all epithelial and fibroblastic lines examined and in some, but not all, hematopoietic cells. -
序列相似性
Belongs to the EPS8 family.
Contains 1 PH domain.
Contains 1 SH3 domain. -
结构域
The SH3 domain mediates interaction with SHB. -
翻译后修饰
Phosphorylated by several receptor tyrosine kinases. - Information by UniProt
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数据库链接
- Entrez Gene: 2059 Human
- Omim: 600206 Human
- SwissProt: Q12929 Human
- Unigene: 591160 Human
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别名
- Epidermal growth factor receptor kinase substrate 8 antibody
- Epidermal growth factor receptor pathway substrate 8 antibody
- EPS 8 antibody
see all
图片
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All lanes : Anti-EPS8 antibody [EPR6112] (ab124882) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : A375 cell lysate
Lane 3 : 293T cell lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 92 kDa -
Staining of EPS8 in Paraffin-embedded Human kidney tissue by Immunohistochemistry using ab124882 at a dilution of 1/100.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab124882 被引用在 2 文献中.
- Lu H et al. Identification of HLA-A*1101-restricted cytotoxic T lymphocyte epitopes derived from epidermal growth factor pathway substrate number 8. Mol Med Rep 14:4999-5006 (2016). WB ; Human . PubMed: 27840923
- Tang B et al. Identification of human leukemia antigen A*0201-restricted epitopes derived from epidermal growth factor pathway substrate number 8. Mol Med Rep 12:1741-52 (2015). PubMed: 25936538