The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Detects a band of approximately 97 kDa (predicted molecular weight: 92 kDa).
1/100 - 1/250. antigen retrieval is recommended.
1/50 - 1/100.
Is unsuitable for Flow Cyt or IP.
Upon binding to EGF receptor enhances EGF-dependent mitogenic signals. Can bind multiple cellular targets.
Expressed in all tissues analyzed, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Expressed in all epithelial and fibroblastic lines examined and in some, but not all, hematopoietic cells.
Belongs to the EPS8 family. Contains 1 PH domain. Contains 1 SH3 domain.
The SH3 domain mediates interaction with SHB.
Phosphorylated by several receptor tyrosine kinases.
Lu H et al. Identification of HLA-A*1101-restricted cytotoxic T lymphocyte epitopes derived from epidermal growth factor pathway substrate number 8. Mol Med Rep14:4999-5006 (2016).
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