The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Detects a band of approximately 97 kDa (predicted molecular weight: 92 kDa).
1/100 - 1/250. antigen retrieval is recommended.
1/50 - 1/100.
应用说明Is unsuitable for Flow Cyt or IP.
功能Upon binding to EGF receptor enhances EGF-dependent mitogenic signals. Can bind multiple cellular targets.
组织特异性Expressed in all tissues analyzed, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Expressed in all epithelial and fibroblastic lines examined and in some, but not all, hematopoietic cells.
序列相似性Belongs to the EPS8 family. Contains 1 PH domain. Contains 1 SH3 domain.
结构域The SH3 domain mediates interaction with SHB.
翻译后修饰Phosphorylated by several receptor tyrosine kinases.
Staining of EPS8 in Paraffin-embedded Human kidney tissue by Immunohistochemistry using ab124882 at a dilution of 1/100.
Anti-EPS8 antibody [EPR6112] (ab124882)参考文献
This product has been referenced in:
Lu H et al. Identification of HLA-A*1101-restricted cytotoxic T lymphocyte epitopes derived from epidermal growth factor pathway substrate number 8. Mol Med Rep14:4999-5006 (2016).
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