概述

  • 产品名称
  • 描述
    山羊多克隆抗体to EPS8
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Dog
  • 免疫原

    Synthetic peptide:

    SGVESFDEGSSH

    , corresponding to C terminal amino acids 811-822 of Human EPS8

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline. pH 7.3
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab38329 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution. Preliminary experiments gave no signal but low background in A431 and Hela lysates at up to 1µg/ml.
IHC-P Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Tested in Human Breast Carcinoma showing staining of cancer cells infiltrated in a breast lobe.

靶标

  • 功能
    Upon binding to EGF receptor enhances EGF-dependent mitogenic signals. Can bind multiple cellular targets.
  • 组织特异性
    Expressed in all tissues analyzed, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Expressed in all epithelial and fibroblastic lines examined and in some, but not all, hematopoietic cells.
  • 序列相似性
    Belongs to the EPS8 family.
    Contains 1 PH domain.
    Contains 1 SH3 domain.
  • 结构域
    The SH3 domain mediates interaction with SHB.
  • 翻译后修饰
    Phosphorylated by several receptor tyrosine kinases.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Epidermal growth factor receptor kinase substrate 8 antibody
    • Epidermal growth factor receptor pathway substrate 8 antibody
    • EPS 8 antibody
    • EPS8 antibody
    • EPS8_HUMAN antibody
    see all

图片

  • ab38329 (5µg/ml) staining paraffin embedded Human Breast Carcinoma. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

文献

ab38329 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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