概述

  • 产品名称Anti-EPS15R抗体[EP1147Y]
    参阅全部 EPS15R 一抗
  • 描述
    兔单克隆抗体[EP1147Y] to EPS15R
  • 经测试应用适用于: WB, IP, ICC, Flow Cyt, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthetic peptide corresponding to residues near the C-terminus of human EPS15R was used as an immunogen

  • 阳性对照
    • HeLa cell membrane or nuclear cell lysate Human breast carcinoma tissue
  • 常规说明

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab53006 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/50000. Detects a band of approximately 125 kDa (predicted molecular weight: 94 kDa).
IP 1/70.
ICC 1/50 - 1/100.
Flow Cyt 1/10.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P Use at an assay dependent concentration.

靶标

  • 功能Seems to be a constitutive component of clathrin-coated pits that is required for receptor-mediated endocytosis. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR); internalization of ITGB1 as DAB2-dependent cargo but not TFR seems to require association with DAB2.
  • 序列相似性Contains 1 EF-hand domain.
    Contains 3 EH domains.
  • 翻译后修饰Phosphorylated on tyrosine residues by EGFR.
  • 细胞定位Cell membrane. Nucleus. Membrane > coated pit. Localized to plasma membrane coated pits.
  • Information by UniProt
  • 数据库链接
  • 别名
    • EP15R_HUMAN antibody
    • epidermal growth factor receptor pathway substrate 15- ike 1 antibody
    • Epidermal growth factor receptor substrate 15 like 1 antibody
    • Epidermal growth factor receptor substrate 15-like 1 antibody
    • epidermal growth factor receptor substrate EPS15R antibody
    • Eps15 related protein antibody
    • Eps15-related protein antibody
    • EPS15L1 antibody
    • Eps15R antibody
    see all

Anti-EPS15R antibody [EP1147Y] 图像

  • Anti-EPS15R antibody [EP1147Y] (ab53006) at 1/50000 dilution + HeLa cell lysate at 10 µg

    Secondary
    goat anti-rabbit HRP labelled at 1/2000 dilution

    Predicted band size : 94 kDa
    Observed band size : 125 kDa (why is the actual band size different from the predicted?)
  • ab53006, at a 1/50 dilution, staining Human EPS15R in Breast Carcinoma, using Immunohistochemsitry, Paraffin embedded tissue.

Anti-EPS15R antibody [EP1147Y] (ab53006)参考文献

This product has been referenced in:
  • Liu Z & Zheng Y A requirement for epsin in mitotic membrane and spindle organization. J Cell Biol 186:473-80 (2009). Read more (PubMed: 19704019) »
  • Chen C & Zhuang X Epsin 1 is a cargo-specific adaptor for the clathrin-mediated endocytosis of the influenza virus. Proc Natl Acad Sci U S A 105:11790-5 (2008). Read more (PubMed: 18689690) »

See all 2 Publications for this product

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