Anti-Epoxide hydrolase抗体(ab96695)
Key features and details
- Rabbit polyclonal to Epoxide hydrolase
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Epoxide hydrolase抗体
参阅全部 Epoxide hydrolase 一抗 -
描述
兔多克隆抗体to Epoxide hydrolase -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow, Pig -
免疫原
Recombinant fragment, corresponding to a region within amino acids 1-193 of Human Epoxide hydrolase (NP_000111).
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阳性对照
- WB: 293T, HeLa and HepG2 cell lysates ICC/IF: Hela cells IHC-P: SG xenograft
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab96695于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 53 kDa.
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IHC-P |
1/100 - 1/500.
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ICC/IF |
1/100 - 1/200.
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 53 kDa. |
IHC-P
1/100 - 1/500. |
ICC/IF
1/100 - 1/200. |
靶标
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功能
Biotransformation enzyme that catalyzes the hydrolysis of arene and aliphatic epoxides to less reactive and more water soluble dihydrodiols by the trans addition of water. -
组织特异性
Found in liver. -
疾病相关
Note=In some populations, the high activity haplotype tyr113/his139 is overrepresented among women suffering from pregnancy-induced hypertension (pre-eclampsia) when compared with healthy controls.
Defects in EPHX1 are a cause of familial hypercholanemia (FHCA) [MIM:607748]. FHCA is a disorder characterized by elevated serum bile acid concentrations, itching, and fat malabsorption. -
序列相似性
Belongs to the peptidase S33 family. -
细胞定位
Microsome membrane. Endoplasmic reticulum membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 535293 Cow
- Entrez Gene: 2052 Human
- Entrez Gene: 13849 Mouse
- Entrez Gene: 397639 Pig
- Entrez Gene: 25315 Rat
- Omim: 132810 Human
- SwissProt: P07099 Human
- SwissProt: Q9D379 Mouse
see all -
别名
- EPHX 1 antibody
- EPHX antibody
- Ephx1 antibody
see all
图片
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All lanes : Anti-Epoxide hydrolase antibody (ab96695) at 1/1000 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 53 kDa
10% SDS PAGE -
ab96695, at a 1/200 dilution, staining Epoxide hydrolase in methanol fixed HeLa cells by Immunofluorescence analysis. Lower image costained with Hoerchst 33342.
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ab96695, at a 1/500 dilution, staining Epoxide hydrolase in paraffin embedded SG xenograft by Immunohistochemical analysis.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab96695 被引用在 4 文献中.
- González-Alvarez ME et al. Obesity alters the ovarian proteomic response to zearalenone exposure†. Biol Reprod 105:278-289 (2021). PubMed: 33855340
- Cheng H et al. Emerging role of EPHX1 in chemoresistance of acute myeloid leukemia by regurlating drug-metabolizing enzymes and apoptotic signaling. Mol Carcinog N/A:N/A (2019). PubMed: 30644597
- Kumar M et al. Insulin activates intracellular transport of lipid droplets to release triglycerides from the liver. J Cell Biol 218:3697-3713 (2019). PubMed: 31604801
- O'Shaughnessy PJ et al. Maternal smoking and fetal sex significantly affect metabolic enzyme expression in the human fetal liver. J Clin Endocrinol Metab 96:2851-60 (2011). WB ; Human . PubMed: 21715529