Anti-Epoxide hydrolase抗体[15B2AD9] (ab110307)

概述

  • 产品名称Anti-Epoxide hydrolase抗体[15B2AD9]
    参阅全部 Epoxide hydrolase 一抗
  • 描述
    小鼠单克隆抗体[15B2AD9] to Epoxide hydrolase
  • 经测试应用适用于: IHC-P, Flow Cyt, ICC/IF, In-Cell ELISA, IPmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Human liver mitochondria.

  • 阳性对照
    • Human HDFn cells Human normal colon FFPE tissue
  • 常规说明

    Product was previously marketed under the MitoSciences sub-brand.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • 存储溶液Preservative: 0.02% Sodium azide
  • Concentration information loading...
  • 纯度>95% by SDS-PAGE
  • 纯化说明The purity of ab110307 is near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
  • 克隆单克隆
  • 克隆编号15B2AD9
  • 同种型IgG1
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab110307 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 10 µg/ml.
In-Cell ELISA Use a concentration of 4 µg/ml. (0.4 µg/well).
IP Use at an assay dependent concentration.

靶标

  • 功能Biotransformation enzyme that catalyzes the hydrolysis of arene and aliphatic epoxides to less reactive and more water soluble dihydrodiols by the trans addition of water.
  • 组织特异性Found in liver.
  • 疾病相关Note=In some populations, the high activity haplotype tyr113/his139 is overrepresented among women suffering from pregnancy-induced hypertension (pre-eclampsia) when compared with healthy controls.
    Defects in EPHX1 are a cause of familial hypercholanemia (FHCA) [MIM:607748]. FHCA is a disorder characterized by elevated serum bile acid concentrations, itching, and fat malabsorption.
  • 序列相似性Belongs to the peptidase S33 family.
  • 细胞定位Microsome membrane. Endoplasmic reticulum membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • EPHX 1 antibody
    • EPHX antibody
    • Ephx1 antibody
    • EPOX antibody
    • Epoxide hydratase antibody
    • Epoxide hydrolase 1 antibody
    • Epoxide hydrolase 1 microsomal (xenobiotic) antibody
    • Epoxide hydroxylase 1 antibody
    • Epoxide hydroxylase 1 microsomal (xenobiotic) antibody
    • HYEP_HUMAN antibody
    • HYL1 antibody
    • MEH antibody
    • Microsomal epoxide hydrolase antibody
    see all

Anti-Epoxide hydrolase antibody [15B2AD9] 图像

  • Overlay histogram showing HepG2 cells stained with ab110307 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab110307, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • Immunocytochemistry image of ab110307-stained Human HDFn cells.
    The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15 min). The cells were incubated with ab110307 at 4 µg/ml for 2 hours at room temperature or over night at 4°C. The secondary antibody was (green) Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue).
    Target protein locates mainly in microsomal/ER.
  • IHC image of Epoxide hydrolase staining in Human normal colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab110307, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times

Anti-Epoxide hydrolase antibody [15B2AD9] (ab110307)参考文献

ab110307 has not yet been referenced specifically in any publications.

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