RabMAb

Anti-EPO Receptor (phospho Y485)抗体[EPR2266] (ab79824)

概述

  • 产品名称Anti-EPO Receptor (phospho Y485)抗体[EPR2266]
    参阅全部 EPO Receptor 一抗
  • 描述
    兔单克隆抗体[EPR2266] to EPO Receptor (phospho Y485)
  • 特异性ab79824 only detects EPO Receptor phosphorylated at Tyrosine 485.
  • 经测试应用适用于: WB, IPmore details
    不适用于: Flow Cyt,ICC or IHC-P
  • 种属反应性
    与反应: Human
  • 免疫原

    A phospho specific peptide corresponding to residues surrounding Tyrosine 485 of human EPO Receptor.

  • 阳性对照
    • MCF-7 cell lysate treated with pervanadate.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab79824 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/50000. Detects a band of approximately 59 kDa (predicted molecular weight: 55 kDa).
IP 1/20.
  • 应用说明Is unsuitable for Flow Cyt,ICC or IHC-P.
  • 靶标

    • 功能Receptor for erythropoietin. Mediates erythropoietin-induced erythroblast proliferation and differentiation. Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3. May also activate the LYN tyrosine kinase.
      Isoform EPOR-T acts as a dominant-negative receptor of EPOR-mediated signaling.
    • 组织特异性Erythroid cells and erythroid progenitor cells. Isoform EPOR-F is the most abundant form in EPO-dependent erythroleukemia cells and in late-stage erythroid progenitors. Isoform EPOR-S and isoform EPOR-T are the predominant forms in bone marrow. Isoform EPOR-T is the most abundant from in early-stage erythroid progenitor cells.
    • 疾病相关Defects in EPOR are the cause of erythrocytosis familial type 1 (ECYT1) [MIM:133100]. ECYT1 is an autosomal dominant disorder characterized by increased serum red blood cell mass, elevated hemoglobin and hematocrit, hypersensitivity of erythroid progenitors to erythropoietin, erythropoietin low serum levels, and no increase in platelets nor leukocytes. It has a relatively benign course and does not progress to leukemia.
    • 序列相似性Belongs to the type I cytokine receptor family. Type 1 subfamily.
      Contains 1 fibronectin type-III domain.
    • 结构域The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
      The box 1 motif is required for JAK interaction and/or activation.
      Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
    • 翻译后修饰On EPO stimulation, phosphorylated on C-terminal tyrosine residues by JAK2. The phosphotyrosine motifs are also recruitment sites for several SH2-containing proteins and adapter proteins which mediate cell proliferation. Phosphorylation on Tyr-454 is required for PTPN6 interaction, Tyr-426 for PTPN11. Tyr-426 is also required for SOCS3 binding, but Tyr-454/Tyr-456 motif is the preferred binding site.
      Ubiquitinated by NOSIP; appears to be either multi-monoubiquitinated or polyubiquitinated. Ubiquitination mediates proliferation and survival of EPO-dependent cells.
    • 细胞定位Cell membrane and Secreted. Secreted and located to the cell surface.
    • Information by UniProt
    • 数据库链接
    • 别名
      • EPO R antibody
      • EPO-R antibody
      • epor antibody
      • EPOR_HUMAN antibody
      • Erythropoietin receptor antibody
      • Erythropoietin receptor precursor antibody
      • MGC138358 antibody
      see all

    Anti-EPO Receptor (phospho Y485) antibody [EPR2266] 图像

    • All lanes : Anti-EPO Receptor (phospho Y485) antibody [EPR2266] (ab79824) at 1/50000 dilution

      Lane 1 : MCF-7 cell lysate untreated
      Lane 2 : MCF-7 cell lysate treated with pervanadate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP conjugate at 1/2000 dilution

      Predicted band size : 55 kDa
      Observed band size : 59 kDa (why is the actual band size different from the predicted?)

    Anti-EPO Receptor (phospho Y485) antibody [EPR2266] (ab79824)参考文献

    This product has been referenced in:
    • Chen AY  et al. Productive parvovirus B19 infection of primary human erythroid progenitor cells at hypoxia is regulated by STAT5A and MEK signaling but not HIFa. PLoS Pathog 7:e1002088 (2011). WB . Read more (PubMed: 21698228) »
    • Cao Y  et al. Erythropoietin Receptor Signaling Through STAT3 Is Required For Glioma Stem Cell Maintenance. Genes Cancer 1:50-61 (2010). Read more (PubMed: 20657792) »

    See all 2 Publications for this product

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