Anti-EPO Receptor抗体[MM-0031-6G7] (ab56310)


  • 产品名称Anti-EPO Receptor抗体[MM-0031-6G7]
    参阅全部 EPO Receptor 一抗
  • 描述
    小鼠单克隆抗体[MM-0031-6G7] to EPO Receptor
  • 特异性Ab56310 recognizes EPO Receptor using western blot. This antibody has been tested to be able to pick up human EPOR around 60-65 kDa under both reducing and non-reducing conditions. However the immunosignal is much stronger under non-reducing conditions so remove beta-mercaptoethanol or DTT from sample buffer.
  • 经测试应用适用于: IHC-P, WB, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Human recombinant EPO Receptor (extracellular domain)



Our Abpromise guarantee covers the use of ab56310 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/10 - 1/40. Proteinase k digestion is required, 20 ug/ml proteinase K (in PBS), digestion at room temperature for 15-30 mins.
WB 1/1000 - 1/2000. Use under non reducing condition. Detects a band of approximately 60 kDa (predicted molecular weight: 55 kDa).
Flow Cyt Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.


  • 功能Receptor for erythropoietin. Mediates erythropoietin-induced erythroblast proliferation and differentiation. Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3. May also activate the LYN tyrosine kinase.
    Isoform EPOR-T acts as a dominant-negative receptor of EPOR-mediated signaling.
  • 组织特异性Erythroid cells and erythroid progenitor cells. Isoform EPOR-F is the most abundant form in EPO-dependent erythroleukemia cells and in late-stage erythroid progenitors. Isoform EPOR-S and isoform EPOR-T are the predominant forms in bone marrow. Isoform EPOR-T is the most abundant from in early-stage erythroid progenitor cells.
  • 疾病相关Defects in EPOR are the cause of erythrocytosis familial type 1 (ECYT1) [MIM:133100]. ECYT1 is an autosomal dominant disorder characterized by increased serum red blood cell mass, elevated hemoglobin and hematocrit, hypersensitivity of erythroid progenitors to erythropoietin, erythropoietin low serum levels, and no increase in platelets nor leukocytes. It has a relatively benign course and does not progress to leukemia.
  • 序列相似性Belongs to the type I cytokine receptor family. Type 1 subfamily.
    Contains 1 fibronectin type-III domain.
  • 结构域The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.
    Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
  • 翻译后修饰On EPO stimulation, phosphorylated on C-terminal tyrosine residues by JAK2. The phosphotyrosine motifs are also recruitment sites for several SH2-containing proteins and adapter proteins which mediate cell proliferation. Phosphorylation on Tyr-454 is required for PTPN6 interaction, Tyr-426 for PTPN11. Tyr-426 is also required for SOCS3 binding, but Tyr-454/Tyr-456 motif is the preferred binding site.
    Ubiquitinated by NOSIP; appears to be either multi-monoubiquitinated or polyubiquitinated. Ubiquitination mediates proliferation and survival of EPO-dependent cells.
  • 细胞定位Cell membrane and Secreted. Secreted and located to the cell surface.
  • Information by UniProt
  • 数据库链接
  • 别名
    • EPO R antibody
    • EPO-R antibody
    • epor antibody
    • EPOR_HUMAN antibody
    • Erythropoietin receptor antibody
    • Erythropoietin receptor precursor antibody
    • MGC138358 antibody
    see all

Anti-EPO Receptor antibody [MM-0031-6G7] 图像

  • Formalin fixed and Paraffin embedded human placenta tissue section was subjected to IHC staining using ab56310 (Proteinase K pretreatment is required).
  • Overlay histogram showing K562 cells stained with ab56310 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab56310, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Anti-EPO Receptor antibody [MM-0031-6G7] (ab56310)参考文献

This product has been referenced in:
  • McGraw KL  et al. Erythropoietin receptor signaling is membrane raft dependent. PLoS One 7:e34477 (2012). WB ; Human . Read more (PubMed: 22509308) »
  • Chen AY  et al. Productive Parvovirus B19 Infection of Primary Human Erythroid Progenitor Cells at Hypoxia Is Regulated by STAT5A and MEK Signaling but not HIFa. PLoS Pathog 7:e1002088 (2011). WB, Flow Cyt ; Human . Read more (PubMed: 21698228) »

See all 4 Publications for this product

Product Wall

Thank you for contacting us.

According to the datasheet, we have used a human recombinant EPO Receptor (extracellular domain) as the immunogen. As this protein is a single-pass type I membrane protein, the immunogen can be found within range...

Read More

Vielen Dank für Ihre Anfrage. Die Frau xxxxx ist zur Zeit außer Haus. Ich werde daher versuchen Ihre Fragen so gut wie möglich zu beantworten.

Ihr Plan mit einer Verdünnung von 1/100 und ohne antigen retrival anzufangen h&...

Read More


ab54659: 100%ABR-00000

ab56310: 100%ABR-00000

Ablaufdatum: 01. Februar 2013

Es freut mich zu hören, dass Sie unser Angebot die Antikörper ab54659 und ab56310 inKaninchen z...

Read More

Vielen Dank für Ihren Vorschlag.

Die Homologie des Immunogen von ab56310 mit Maus liegt bei 81%.

Das ist eher grenzwertig für einen monoklonalen Antikörper.

Ich stimme Ihnen dennoch zu, dass es wahrscheinlic...

Read More

Thank you for contacting us.

These antibodies haven't yet been tested in ICC/IF, this however does not mean these can not be used.

We can provide 100% Abreview testing discount if the customer is interested in testing any of these an...

Read More