Anti-EPO Receptor抗体(ab54659)

概述

  • 产品名称Anti-EPO Receptor抗体
    参阅全部 EPO Receptor 一抗
  • 描述
    小鼠单克隆抗体to EPO Receptor
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment: PDPKFESKAA LLAARGPEEL LCFTERLEDL VCFWEEAASA GVGPGNYSFS YQLEDEPWKL CRLHQAPTAR GAVRFWCSLP TADTSSFVPL ELRVTAASGA , corresponding to amino acids 31-131 of Human EPO Receptor

性能

应用

Our Abpromise guarantee covers the use of ab54659 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明WB: Use at a concentration of 1-5 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Receptor for erythropoietin. Mediates erythropoietin-induced erythroblast proliferation and differentiation. Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3. May also activate the LYN tyrosine kinase.
      Isoform EPOR-T acts as a dominant-negative receptor of EPOR-mediated signaling.
    • 组织特异性Erythroid cells and erythroid progenitor cells. Isoform EPOR-F is the most abundant form in EPO-dependent erythroleukemia cells and in late-stage erythroid progenitors. Isoform EPOR-S and isoform EPOR-T are the predominant forms in bone marrow. Isoform EPOR-T is the most abundant from in early-stage erythroid progenitor cells.
    • 疾病相关Defects in EPOR are the cause of erythrocytosis familial type 1 (ECYT1) [MIM:133100]. ECYT1 is an autosomal dominant disorder characterized by increased serum red blood cell mass, elevated hemoglobin and hematocrit, hypersensitivity of erythroid progenitors to erythropoietin, erythropoietin low serum levels, and no increase in platelets nor leukocytes. It has a relatively benign course and does not progress to leukemia.
    • 序列相似性Belongs to the type I cytokine receptor family. Type 1 subfamily.
      Contains 1 fibronectin type-III domain.
    • 结构域The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
      The box 1 motif is required for JAK interaction and/or activation.
      Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
    • 翻译后修饰On EPO stimulation, phosphorylated on C-terminal tyrosine residues by JAK2. The phosphotyrosine motifs are also recruitment sites for several SH2-containing proteins and adapter proteins which mediate cell proliferation. Phosphorylation on Tyr-454 is required for PTPN6 interaction, Tyr-426 for PTPN11. Tyr-426 is also required for SOCS3 binding, but Tyr-454/Tyr-456 motif is the preferred binding site.
      Ubiquitinated by NOSIP; appears to be either multi-monoubiquitinated or polyubiquitinated. Ubiquitination mediates proliferation and survival of EPO-dependent cells.
    • 细胞定位Cell membrane and Secreted. Secreted and located to the cell surface.
    • Information by UniProt
    • 数据库链接
    • 别名
      • EPO R antibody
      • EPO-R antibody
      • epor antibody
      • EPOR_HUMAN antibody
      • Erythropoietin receptor antibody
      • Erythropoietin receptor precursor antibody
      • MGC138358 antibody
      see all

    Anti-EPO Receptor antibody 图像



    • Predicted band size : 55 kDa
      EPO Receptor antibody (ab54659) at 1ug/lane + HeLa cell lysate at 25ug/lane.

    Anti-EPO Receptor antibody (ab54659)参考文献

    ab54659 has not yet been referenced specifically in any publications.

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    DISCOUNT CODEs:

    ab54659: 100%ABR-00000

    ab56310: 100%ABR-00000


    Ablaufdatum: 01. Februar 2013



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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"