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Our Abpromise guarantee covers the use of ab30545 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use a concentration of 0.5 - 1 µg/ml. Detection limit is ~1ng/well.|
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 21 kDa.|
|Neutralising||Use a concentration of 1 - 3 µg/ml. 1 hour at 37°C. The suggested antibody concentration can detect 0.3 units/ml recombinant human EPO in cell line TF1.|
|IHC-P||Use at an assay dependent concentration.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"