• 产品名称Anti-EPLIN抗体
    参阅全部 EPLIN 一抗
  • 描述
    兔多克隆抗体to EPLIN
  • 特异性This antibody reacts with EPLIN
  • 经测试应用适用于: ICC/IF, WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Cow, Dog, Pig
  • 免疫原

    A region within synthetic peptide: GVLAASMEAK ASSQQEKEDK PAETKKLRIA WPPPTELGSS GSALEEGIKM, corresponding to amino acids 502-551 of Human EPLIN

  • 阳性对照
    • Placenta lysate



Our Abpromise guarantee covers the use of ab50196 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/100.
WB Use a concentration of 2 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 85 kDa).Can be blocked with Human EPLIN peptide (ab111676). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/12500.


  • 功能Binds to actin monomers and filaments. Increases the number and size of actin stress fibers and inhibits membrane ruffling. Inhibits actin filament depolymerization. Bundles actin filaments, delays filament nucleation and reduces formation of branched filaments.
  • 组织特异性Highly expressed in placenta, kidney, pancreas, prostate, ovary, spleen and heart. Also detected in lung, liver, brain, skeletal muscle, thymus, testis and intestine. Not detected in leukocytes. Isoform Beta expressed generally at very low levels. Isoform Alpha abundant in epithelial cells from mammary gland, prostate and in normal oral keratinocytes. Low levels in aortic endothelial cells and dermal fibroblasts. Not detectable in myocardium.
  • 序列相似性Contains 1 LIM zinc-binding domain.
  • 结构域Contains at least 2 actin-binding domains, one on each side of the LIM domain. Both domains bind actin monomers and filaments. The C-terminal domain binds filaments more efficiently than the N-terminus.
  • 细胞定位Cytoplasm. Cell junction > focal adhesion. Cytoplasm > cytoskeleton. This cytoskeletal protein co-localizes with actin stress fibers and focal adhesion plaques.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 1110021C24Rik antibody
    • Epithelial protein lost in neoplasm antibody
    • Epithelial protein lost in neoplasm beta antibody
    • EPLIN antibody
    • FLJ38853 antibody
    • LIM domain and actin binding 1 antibody
    • LIM domain and actin binding protein 1 antibody
    • LIM domain and actin-binding protein 1 antibody
    • LIMA1 antibody
    • LIMA1_HUMAN antibody
    • MGC131726 antibody
    • SREBP3 antibody
    • Sterol regulatory element binding protein 3 antibody
    see all

Anti-EPLIN antibody 图像

  • Anti-EPLIN antibody (ab50196) at 2 µg/ml + Placental lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 85 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)

Anti-EPLIN antibody (ab50196)参考文献

ab50196 has not yet been referenced specifically in any publications.

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Thank you for your email. I have checked the protocol you have kindly sent and would like to make few suggestions that I am sure will help to get staining at right location; - Use 5% BSA blocking solution for 1-1.5 hours. - Try using more dil...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Kidney)
Specification Kidney
Fixative Acetone
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 20°C

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Verified customer

提交于 Nov 29 2010