概述
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产品名称Anti-EPLIN抗体
参阅全部 EPLIN 一抗 -
描述兔多克隆抗体to EPLIN
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宿主Rabbit
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特异性This antibody reacts with EPLIN
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经测试应用适用于: ICC/IF, WB, ELISAmore details
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种属反应性与反应: Human
预测可用于: Mouse, Cow, Dog, Pig
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免疫原
A region within synthetic peptide: GVLAASMEAK ASSQQEKEDK PAETKKLRIA WPPPTELGSS GSALEEGIKM, corresponding to amino acids 502-551 of Human EPLIN
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阳性对照
- Placenta lysate
性能
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形式Liquid
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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存储溶液Preservative: None
Constituents: 2% Sucrose, PBS -
Concentration information loading... -
纯度Immunogen affinity purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
应用
Our Abpromise guarantee covers the use of ab50196 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | 1/100. | |
| WB | Use a concentration of 2 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 85 kDa).Can be blocked with Human EPLIN peptide (ab111676). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. | |
| ELISA | 1/12500. |
靶标
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功能Binds to actin monomers and filaments. Increases the number and size of actin stress fibers and inhibits membrane ruffling. Inhibits actin filament depolymerization. Bundles actin filaments, delays filament nucleation and reduces formation of branched filaments.
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组织特异性Highly expressed in placenta, kidney, pancreas, prostate, ovary, spleen and heart. Also detected in lung, liver, brain, skeletal muscle, thymus, testis and intestine. Not detected in leukocytes. Isoform Beta expressed generally at very low levels. Isoform Alpha abundant in epithelial cells from mammary gland, prostate and in normal oral keratinocytes. Low levels in aortic endothelial cells and dermal fibroblasts. Not detectable in myocardium.
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序列相似性Contains 1 LIM zinc-binding domain.
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结构域Contains at least 2 actin-binding domains, one on each side of the LIM domain. Both domains bind actin monomers and filaments. The C-terminal domain binds filaments more efficiently than the N-terminus.
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细胞定位Cytoplasm. Cell junction > focal adhesion. Cytoplasm > cytoskeleton. This cytoskeletal protein co-localizes with actin stress fibers and focal adhesion plaques.
- Information by UniProt
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数据库链接
- Entrez Gene: 540637 Cow
- Entrez Gene: 51474 Human
- Entrez Gene: 65970 Mouse
- Omim: 608364 Human
- SwissProt: Q9UHB6 Human
- SwissProt: Q9ERG0 Mouse
- Unigene: 525419 Human
- Unigene: 33207 Mouse
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别名
- 1110021C24Rik antibody
- Epithelial protein lost in neoplasm antibody
- Epithelial protein lost in neoplasm beta antibody
see all
图片
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Western blot - Anti-EPLIN antibody (ab50196)Anti-EPLIN antibody (ab50196) at 2 µg/ml + Placental lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 85 kDa
Observed band size: 90 kDa (why is the actual band size different from the predicted?)
文献
ab50196 has not yet been referenced specifically in any publications.
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"