Anti-EPLIN抗体(ab50196)

Abreviews (1)Q&A (2)
Customer reviews and FAQs

概述

  • 产品名称
    Anti-EPLIN抗体
    参阅全部 EPLIN 一抗
  • 描述
    兔多克隆抗体to EPLIN
  • 特异性
    This antibody reacts with EPLIN
  • 经测试应用
    适用于: ICC/IF, WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Cow, Dog, Pig
  • 免疫原

    A region within synthetic peptide: GVLAASMEAK ASSQQEKEDK PAETKKLRIA WPPPTELGSS GSALEEGIKM, corresponding to amino acids 502-551 of Human EPLIN

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照
    • Placenta lysate

性能

应用

Our Abpromise guarantee covers the use of ab50196 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/100.
WB Use a concentration of 2 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 85 kDa).Can be blocked with Human EPLIN peptide (ab111676). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/12500.

靶标

  • 功能
    Binds to actin monomers and filaments. Increases the number and size of actin stress fibers and inhibits membrane ruffling. Inhibits actin filament depolymerization. Bundles actin filaments, delays filament nucleation and reduces formation of branched filaments.
  • 组织特异性
    Highly expressed in placenta, kidney, pancreas, prostate, ovary, spleen and heart. Also detected in lung, liver, brain, skeletal muscle, thymus, testis and intestine. Not detected in leukocytes. Isoform Beta expressed generally at very low levels. Isoform Alpha abundant in epithelial cells from mammary gland, prostate and in normal oral keratinocytes. Low levels in aortic endothelial cells and dermal fibroblasts. Not detectable in myocardium.
  • 序列相似性
    Contains 1 LIM zinc-binding domain.
  • 结构域
    Contains at least 2 actin-binding domains, one on each side of the LIM domain. Both domains bind actin monomers and filaments. The C-terminal domain binds filaments more efficiently than the N-terminus.
  • 细胞定位
    Cytoplasm. Cell junction > focal adhesion. Cytoplasm > cytoskeleton. This cytoskeletal protein co-localizes with actin stress fibers and focal adhesion plaques.
  • Information by UniProt
  • 数据库链接
    • 别名
      • 1110021C24Rik antibody
      • Epithelial protein lost in neoplasm antibody
      • Epithelial protein lost in neoplasm beta antibody
      • EPLIN antibody
      • FLJ38853 antibody
      • LIM domain and actin binding 1 antibody
      • LIM domain and actin binding protein 1 antibody
      • LIM domain and actin-binding protein 1 antibody
      • LIMA1 antibody
      • LIMA1_HUMAN antibody
      • MGC131726 antibody
      • SREBP3 antibody
      • Sterol regulatory element binding protein 3 antibody
      see all

    Anti-EPLIN antibody 图像

    • Western blot - EPLIN antibody (ab50196)
      Western blot - EPLIN antibody (ab50196)
      Anti-EPLIN antibody (ab50196) at 2 µg/ml + Placental lysate at 10 µg

      Secondary
      HRP conjugated anti-Rabbit IgG at 1/50000 dilution

      Predicted band size : 85 kDa
      Observed band size : 90 kDa (why is the actual band size different from the predicted?)

    Anti-EPLIN antibody (ab50196)参考文献

    ab50196 has not yet been referenced specifically in any publications.

    发表研究结果有使用 ab50196？请让我们知道，以便我们可以引用本数据表中的参考文章。

    Product Wall

    提交 ab50196 的 abreview 提交 ab50196 的问题

    3 Abreviews or Q&A

    10
    Votes: Highest First

    Hi, I have attached the questionnaire form for these two antibodies. Hope this is helpful and you can recommend further steps to improve the function of the antibody. Regarding the other antibodies in the same order, there are a few antibodies we are s...

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    Thank you for your email. I have checked the protocol you have kindly sent and would like to make few suggestions that I am sure will help to get staining at right location; - Use 5% BSA blocking solution for 1-1.5 hours. - Try using more dil...

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    Hi,  I am writing regarding the ORDER #933686. The following antibodies from that order do not seem to work as specified: EPLIN Cat. No. ab50196 ZO1 tight junction protein Cat. No. ab59720 Both proteins have been shown to localize at ...

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    Thank you for contacting us. I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly. I am atta...

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    Immunocytochemistry/ Immunofluorescence abreview for Anti-EPLIN antibody

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Kidney)
    Specification
    Kidney
    Fixative
    Acetone
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 20°C
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    Username

    晴子 鶴見

    Verified customer

    提交于 Nov 29 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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