Anti-Epithelial Antigen抗体[Ber-EP4], prediluted (ab82752)

概述

  • 产品名称Anti-Epithelial Antigen抗体[Ber-EP4], prediluted
  • 描述
    小鼠单克隆抗体[Ber-EP4] to Epithelial Antigen, prediluted
  • 经测试应用适用于: IHC-P, IHC-Fr, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Human breast carcinoma cell line MCF-7.

  • 阳性对照
    • Kidney.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab82752 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/1. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr 1/1. Fix with acetone.
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 相关性Epithelial antigen is a membrane bound, glycosylated phosphoprotein. The protein is anchored to the apical surface of many epithelia by a transmembrane domain, with the degree of glycosylation varying with cell type. It may may play a role in adhesive functions and in cell to cell interactions, metastasis and signalling and provide a protective layer on epithelial surfaces. It is capable of direct or indirect interaction with actin cytoskeleton.
  • 细胞定位Apical cell membrane; Single pass type I membrane protein. Note: Exclusively located in the apical domain of the plasma membrane of highly polarized epithelial cells. After endocytosis, internalized and recycled to the cell membrane. Located to microvilli and to the tips of long filopodial protusions.
  • 数据库链接
  • 别名
    • Breast carcinoma associated antigen DF3 antibody
    • Carcinoma associated mucin antibody
    • CD227 antigen antibody
    • EMA antibody
    • Episialin antibody
    • H23AG antibody
    • MUC1 antibody
    • Mucin 1 transmembrane antibody
    • Peanut reactive urinary mucin antibody
    • PEM antibody
    • PEMT antibody
    • Polymorphic epithelial mucin antibody
    • PUM antibody
    • Tumor associated epithelial membrane antigen antibody
    • Tumor associated mucin antibody
    see all

Anti-Epithelial Antigen antibody [Ber-EP4], prediluted 图像

  • ICC/IF image of ab82752 stained MCF7 cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab82752, 1ml) overnight at +4°C. The secondary antibody (green) was ab96879 Dylight 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Epithelial Antigen antibody [Ber-EP4], prediluted (ab82752)参考文献

This product has been referenced in:
  • Beer TW  et al. Ber EP4 and epithelial membrane antigen aid distinction of basal cell, squamous cell and basosquamous carcinomas of the skin. Histopathology 37:218-23 (2000). IHC-P, IHC-Fr ; Human . Read more (PubMed: 10971697) »

See 1 Publication for this product

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