概述

  • 产品名称Anti-Ephrin B3抗体
    参阅全部 Ephrin B3 一抗
  • 描述
    兔多克隆抗体to Ephrin B3
  • 经测试应用适用于: ICC/IF, WB, IHCmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Horse, Cow, Cat, Dog, Pig, Macaque Monkey
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human Ephrin B3.

  • 阳性对照
    • This antibody gave a positive signal in the following tissue lysates: Human brain; Mouse brain; Rat brain; Mouse forebrain; Rat forebrain; Mouse Embryonic brain E10; Mouse embryonic brain E14.

性能

应用

Our Abpromise guarantee covers the use of ab101699 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 36 kDa).
IHC Use at an assay dependent concentration. PubMed: 25309991

靶标

  • 功能May play a pivotal role in forebrain function. Binds to, and induce the collapse of, commissural axons/growth cones in vitro. May play a role in constraining the orientation of longitudinally projecting axons.
  • 组织特异性Highly expressed in brain; expressed in embryonic floor plate, roof plate and hindbrain segments.
  • 序列相似性Belongs to the ephrin family.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • EFL 6 antibody
    • EFL6 antibody
    • EFNB 3 antibody
    • Efnb3 antibody
    • EFNB3_HUMAN antibody
    • EPH related receptor transmembrane ligand ELK L3 antibody
    • EPH related receptor transmembrane ligand ELKL3 antibody
    • EPH related receptor tyrosine kinase ligand 8 antibody
    • EPH-related receptor transmembrane ligand ELK-L3 antibody
    • EPH-related receptor tyrosine kinase ligand 8 antibody
    • Ephrin B3 antibody
    • Ephrin-B3 antibody
    • EphrinB3 antibody
    • EPLG 8 antibody
    • EPLG8 antibody
    • LERK 8 antibody
    • LERK-8 antibody
    • LERK8 antibody
    see all

Anti-Ephrin B3 antibody 图像

  • All lanes : Anti-Ephrin B3 antibody (ab101699) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Brain (Mouse) Tissue Lysate
    Lane 3 : Brain (Rat) Tissue Lysate
    Lane 4 : Forebrain (Mouse) Tissue Lysate
    Lane 5 : Rat Forebrain
    Lane 6 : E10 Mouse Embryo Brain Tissue Lysate
    Lane 7 : E14 Mouse Embryo Brain Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 36 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)


    Exposure time : 90 seconds
  • ICC/IF image of ab101699 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab101699, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Ephrin B3 antibody (ab101699)参考文献

This product has been referenced in:
  • Qu B  et al. Testing stem cell therapy in a rat model of inflammatory bowel disease: role of bone marrow stem cells and stem cell factor in mucosal regeneration. PLoS One 9:e107891 (2014). IHC ; Rat . Read more (PubMed: 25309991) »

See 1 Publication for this product

Product Wall

DISCOUNT CODES: 100%ABR-E7TFV for testing ab89461 in Zebrafish 100%ABR-E7TF3 for testing ab67168 in Zebrafish 100%ABR-E7TFM for testing ab101699 in Zebrafish Expiration date for all the three codes: 22. February 2012 I am very pleased to hear you would...

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Thank you very much for your inquiry. Indeed, it is difficult to find antibodies crossreactive with Zebrafish. I don't know whether I have already told you about our list of Zebrafish crossreactive antibodies? It can be found under the following link: ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"