Anti-Ephrin B2抗体(ab96264)


  • 产品名称Anti-Ephrin B2抗体
    参阅全部 Ephrin B2 一抗
  • 描述
    兔多克隆抗体to Ephrin B2
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rat
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human Ephrin B2.

  • 阳性对照
    • This antibody gave a positive signal in Human lung and Human kidney tissue lysates.



Our Abpromise guarantee covers the use of ab96264 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 36 kDa).
ICC/IF Use a concentration of 5 µg/ml.


  • 功能Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds to receptor tyrosine kinase including EPHA4, EPHA3 and EPHB4. Together with EPHB4 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells. May play a role in constraining the orientation of longitudinally projecting axons.
    (Microbial infection) Acts as a receptor for Hendra virus and Nipah virus.
  • 组织特异性Lung and kidney.
  • 序列相似性Belongs to the ephrin family.
    Contains 1 ephrin RBD (ephrin receptor-binding) domain.
  • 翻译后修饰Inducible phosphorylation of tyrosine residues in the cytoplasmic domain.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • EFN B2 antibody
    • EFNB 2 antibody
    • Efnb2 antibody
    • EFNB2_HUMAN antibody
    • Eph related receptor tyrosine kinase ligand 5 antibody
    • EPH-related receptor tyrosine kinase ligand 5 antibody
    • ephrin B2 antibody
    • Ephrin-B2 antibody
    • EphrinB2 antibody
    • EPLG 5 antibody
    • EPLG5 antibody
    • Htk L antibody
    • HTK ligand antibody
    • HTK-L antibody
    • HTKL antibody
    • LERK 5 antibody
    • LERK-5 antibody
    • LERK5 antibody
    • Ligand of eph related kinase 5 antibody
    • MGC126226 antibody
    • MGC126227 antibody
    • MGC126228 antibody
    • OTTMUSP00000024973 antibody
    see all

Anti-Ephrin B2 antibody 图像

  • All lanes : Anti-Ephrin B2 antibody (ab96264) at 1 µg/ml

    Lane 1 : Lung (Human) Tissue Lysate
    Lane 2 : Human kidney tissue lysate - total protein (ab30203)

    Lysates/proteins at 10 µg per lane.

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 36 kDa
    Observed band size : 32 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 73 kDa,86 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes
  • ICC/IF image of ab96264 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab96264, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Ephrin B2 antibody (ab96264)参考文献

ab96264 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your reply.

I am happy to hear the staining has improved!

The antibody was raised against an extracellular sequence, but since it is a polyclonal it really could have multiple binding epitopes throughout the protein an...

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Thank you for your reply.

Yes, I think you should include the blocking buffer in with the secondary. Typically we recommend 1% BSA, but 5% BSA should be okay.
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Thank you for taking time to contact us. I am sorry to hear that this antibody is not providing satisfactory results.

So the antibody is staining well in immunocytochemistry (ICC/IF)on fixed human endothelial cells? What is the fixative you'...

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Let me just confirm it is the products Anti-Ephrin B2 antibody (ab96264) and Anti-Ephrin B2 (phospho Y316) antibody (ab119323) which you are interested in?

The anti-Ephrin B2 antibody (ab96264) does indeed measure the total Ephrin B2 content,...

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Thank you for calling Abcam earlier today.

I am sorry about the problems you were having with ab96264 in IF. As we discussed, I am sending you a new vial of the antibody to test. Your new order/reference number is *******.

If there ...

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