Anti-Ephrin B (phospho Y330)抗体(ab51118)

概述

  • 产品名称Anti-Ephrin B (phospho Y330)抗体
    参阅全部 Ephrin B 一抗
  • 描述
    兔多克隆抗体to Ephrin B (phospho Y330)
  • 特异性ab51118 detects endogenous levels of Ephrin B only when phosphorylated at tyrosine 330.
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthesized phosphopeptide derived from human Ephrin B around the phosphorylation site of tyrosine 330 (N-I-YP-Y-K).

  • 阳性对照
    • Extracts from 293 cells treated with TNF-a

性能

应用

Our Abpromise guarantee covers the use of ab51118 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
ELISA 1/40000.

靶标

  • 相关性Ephrin B proteins are thought to play key roles in cellular functions as diverse as neuronal migration and blood vessel development. Ephrin B molecules expressed at the membrane surface bind to the Ephrin B family receptors on target cells during cell to cell contact. This interaction leads to cell signaling in the target cell but also generates a reverse signal in the cell expressing Ephrin B on its surface. This reverse signaling event is thought to be critical for vessel maturation and neuronal development. Importantly, tyrosine phosphorylation of Ephrin B is thought to be a critical component of this reverse signaling event. Recent work demonstrated that Tyr331 of Ephrin B was phosphorylated in HEK293 cells after stimulation by the soluble Ephrin B2 receptor tyrosine kinase.
  • 细胞定位Membrane; single pass type I membrane protein.
  • 数据库链接
  • 别名
    • CEK5 ligand antibody
    • EFNB1 antibody
    • ELK L antibody
    • ELK ligand antibody
    • ELKL antibody
    • EPH related receptor tyrosine kinase ligand 2 antibody
    • Ephrin B1 antibody
    • Ephrin B2 antibody
    • LERK2 antibody
    see all

Anti-Ephrin B (phospho Y330) antibody 图像

  • All lanes : Anti-Ephrin B (phospho Y330) antibody (ab51118)

    Lane 1 : extracts from 293 cells treated with TNF-a (20ng/ml, 30min), pre incubated with immunizing peptide
    Lane 2 : extracts from 293 cells treated with TNF-a (20ng/ml, 30min)


    Predicted band size : 38 kDa

Anti-Ephrin B (phospho Y330) antibody (ab51118)参考文献

ab51118 has not yet been referenced specifically in any publications.

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