概述

  • 产品名称
    Anti-Ephrin A5抗体
    参阅全部 Ephrin A5 一抗
  • 描述
    兔多克隆抗体to Ephrin A5
  • 特异性
    ab70114 detects endogenous levels of total Ephrin A5 protein.
  • 经测试应用
    适用于: Flow Cyt, IHC-Fr, WB, ELISA, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Synthetic peptide derived from an internal sequence of human Ephrin A5.

  • 阳性对照
    • A549 cells, HeLa cell extracts and human brain tissue.

性能

应用

Our Abpromise guarantee covers the use of ab70114 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use at an assay dependent concentration. PubMed: 22071104ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration. PubMed: 21628570
WB 1/500 - 1/1000. Detects a band of approximately 24 kDa (predicted molecular weight: 26 kDa).
ELISA 1/40000.
IHC-P 1/50 - 1/100.
ICC/IF 1/500 - 1/1000.

靶标

图片

  • ab70114 staining anti-Ephrin A5 in wild-type and knockout mouse inferior colliculus/cerebellum tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 1% BSA for 15 minutes at 20°C; antigen retrieval was by heat mediation with citric acid. Samples were incubated with primary antibody (1/200 in 1 % TBS/BSA buffer) for 2 hours at 20°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • ab70114 at 1/500 dilution staining Ephrin A5 in A549 cells by Immunofluorescence, in the absence or presence of the immunising peptide.
  • ab70114 at 1/50 dilution staining Ephrin A5 in human brain by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide
  • All lanes : Anti-Ephrin A5 antibody (ab70114) at 1/500 dilution

    Lane 1 : HeLa cell extracts
    Lane 2 : HeLa cell extracts with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 26 kDa
    Observed band size : 24 kDa (why is the actual band size different from the predicted?)

文献

This product has been referenced in:
  • Liu YH  et al. Ascl1 promotes tangential migration and confines migratory routes by induction of Ephb2 in the telencephalon. Sci Rep 7:42895 (2017). Read more (PubMed: 28276447) »
  • Son AI  et al. Formation of persistent hyperplastic primary vitreous in ephrin-A5-/- mice. Invest Ophthalmol Vis Sci 55:1594-606 (2014). Read more (PubMed: 24550361) »

See all 6 Publications for this product

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric Acid
Sample
Mouse Tissue sections (inferior colliculus/cerebellum)
Specification
inferior colliculus/cerebellum
Permeabilization
No
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jun 04 2013

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brainstem)
Specification
Brainstem
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1%
Username

Mr. Carl Hobbs

Verified customer

提交于 Apr 05 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Cerebellum)
Specification
Cerebellum
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

提交于 Mar 18 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (flag-tagged mouse ephrinA5 expressed in CHO cells)
Specification
flag-tagged mouse ephrinA5 expressed in CHO cells
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Jan 03 2013

Thank you very much for providing that information. It makes understanding the problems you have been experiencing with the antibody much easier.

I am sorry that you have not obtained the expected results with this antibody. I'd like to ask a...

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Application
Flow Cytometry
Sample
Human Cell (many normal and tumor cell lines)
Specification
many normal and tumor cell lines
Permeabilization
No
Username

Abcam user community

Verified customer

提交于 Jul 18 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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