概述

  • 产品名称Anti-Ephrin A5抗体
    参阅全部 Ephrin A5 一抗
  • 描述
    小鼠单克隆抗体to Ephrin A5
  • 经测试应用适用于: ELISA, WB, Flow Cyt, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Chicken
  • 免疫原

    Recombinant fragment with tag: FSEKFQLFTP FSLGFEFRPG REYFYISSAI PDNGRRSCLK LKVFVRPTNS CMKTIGVHDR VFDVNDKVEN SLEPADDTVH ESAEPSRGEN , corresponding to amino acids 114-204 of Human Ephrin A5

  • 阳性对照
    • NIH 3T3 whole cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab60705 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use a concentration of 30 µg/ml.
WB Use at an assay dependent concentration. Detects a band of approximately 24 kDa (predicted molecular weight: 26 kDa).
Flow Cyt Use 0.5µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF 1/500.

靶标

Anti-Ephrin A5 antibody 图像

  • Anti-Ephrin A5 antibody (ab60705) + NIH 3T3 whole cell lysate at 50 µg

    Secondary
    Goat Anti-Mouse IgG HRP at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 26 kDa
    Observed band size : 24 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.
  • Overlay histogram showing A549 cells stained with ab60705 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab60705, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
  • Lanes 1 - 3 : Anti Flag tag antibody at 1/500 dilution
    Lanes 4 - 6 : Anti-Ephrin A5 antibody (ab60705) at 1/500 dilution

    Lane 1 : Untransfected CHO cells
    Lane 2 : Flag-tagged ephrin-A5 expressed in CHO cells
    Lane 3 : Flag-tagged ephrin-A5 expressed in CHO cells
    Lane 4 : Untransfected CHO cells
    Lane 5 : Flag-tagged ephrin-A5 expressed in CHO cells
    Lane 6 : Flag-tagged ephrin-A5 expressed in CHO cells

    Lysates/proteins at 10 µg per lane.

    Secondary
    Sheep Polyclonal to anti Rabbit IgG conjugated to HRP at 1/1000 dilution

    Predicted band size : 26 kDa

    This image is courtesy of an anonymous abreview.

    Blocking: 3% milk

    See Abreview

  • ICC/IF image of Ephrin A5 staining on CHO cells using ab60705 (1:500). The CHO cells were tranfected with Flag-tagged mouse Ephrin A5. The cells were fixed using paraformaldehyde and permeabilized using 0.1% Triton. The cells were blocked using 1% BSA for 1 hour at 20°C. ab60705 was diluted 1:500 using 1% BSA in TBS-0.1% Triton and incubated with the cells for 16 hours at 20°C. ab60705 was detected using goat polyclonal to mouse IgG cojugated to Alexa Fluor 488 (1:1000).

    See Abreview

Anti-Ephrin A5 antibody (ab60705)参考文献

This product has been referenced in:
  • Overman JJ  et al. A role for ephrin-A5 in axonal sprouting, recovery, and activity-dependent plasticity after stroke. Proc Natl Acad Sci U S A 109:E2230-9 (2012). WB . Read more (PubMed: 22837401) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (whole brain)
Antigen retrieval step Heat mediated
Permeabilization Yes - Carnoy 12 at 4°C
Specification whole brain
Blocking step BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative paraffin
Username

Abcam user community

Verified customer

提交于 Aug 03 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 20°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric Acid
Sample Mouse Tissue sections (Inferior Colliculus/Cerebellum)
Specification Inferior Colliculus/Cerebellum
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jun 04 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Brainstem)
Specification Brainstem
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

提交于 Apr 11 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Cerebellum)
Specification Cerebellum
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

提交于 Mar 18 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Flag-tagged mouse ephrin-A5 expressed in CHO cells)
Specification Flag-tagged mouse ephrin-A5 expressed in CHO cells
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Jan 03 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Recombinant protein (Flag-tagged ephrin-A5 expressed in CHO cells)
Loading amount 10 µg
Specification Flag-tagged ephrin-A5 expressed in CHO cells
Gel Running Conditions Reduced Denaturing (12.5%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Dec 27 2012

Thank you for your reply which has been forwarded to me as my colleauge Karin is currently away from teh office.

I am sorry that this antibody did not perform as stated on the datasheet. As requested, I have asked our Finance department to i...

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Thank you very much for that information.

If you could possibly provide me with the order number under which ab60705 was received? If you do not have this information could you share the the approximate date of delivery and the delivery addre...

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Thank you very much for providing that information. It makes understanding the problems you have been experiencing with the antibody much easier.

I am sorry that you have not obtained the expected results with this antibody. I'd like to ask a...

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Thank you for your call today and for your questions about our Ephrin A5 antibodies.

Since ab60705has not been tested in ICC/IF, I can offer a discount off a future purchase if you buy ab60705 now, test it inICC/IF and submit feedback to us i...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"