Anti-Ephrin A3抗体(ab64814)


  • 产品名称Anti-Ephrin A3抗体
    参阅全部 Ephrin A3 一抗
  • 描述
    兔多克隆抗体to Ephrin A3
  • 特异性ab64814 detects endogenous levels of total Ephrin A3 protein.
  • 经测试应用适用于: WB, ELISA, Flow Cyt, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    A synthetic peptide derived from the C-terminal of human Ephrin A3.

  • 阳性对照
    • Extracts from HepG2 and Jurkat cells. IF/ICC: HeLa cell line.



Our Abpromise guarantee covers the use of ab64814 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 38 kDa (predicted molecular weight: 26 kDa).
ELISA 1/10000.
Flow Cyt Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 5 mg/ml.


  • 组织特异性Expressed in brain, skeletal muscle, spleen, thymus, prostate, testis, ovary, small intestine, and peripheral blood leukocytes.
  • 序列相似性Belongs to the ephrin family.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • EFL 2 antibody
    • EFL-2 antibody
    • EFL2 antibody
    • EFN A3 antibody
    • EFNA 3 antibody
    • Efna3 antibody
    • EFNA3_HUMAN antibody
    • EHK1 L antibody
    • EHK1 ligand antibody
    • EHK1-L antibody
    • EHK1L antibody
    • EPH related receptor tyrosine kinase ligand 3 antibody
    • EPH-related receptor tyrosine kinase ligand 3 antibody
    • Ephrin-A3 antibody
    • EphrinA3 antibody
    • EPLG 3 antibody
    • EPLG3 antibody
    • LERK 3 antibody
    • LERK-3 antibody
    • LERK3 antibody
    • Ligand of eph related kinase 3 antibody
    see all

Anti-Ephrin A3 antibody 图像

  • ICC/IF image of ab64814 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64814, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-Ephrin A3 antibody (ab64814) at 1/500 dilution

    Lane 1 : Extracts from HepG2 cells
    Lane 2 : Extracts from Jurkat cells
    Lane 3 : Extracts from Jurkat cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.

    Predicted band size : 26 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)

Anti-Ephrin A3 antibody (ab64814)参考文献

ab64814 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (10%)
Sample Mouse Tissue lysate - whole (Brain)
Specification Brain
Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

提交于 May 29 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (Jurkat cell line)
Specification Jurkat cell line
Permeabilization No

Abcam user community

Verified customer

提交于 Jul 22 2011