Anti-Ephrin A2抗体(ab72661)

概述

  • 产品名称Anti-Ephrin A2抗体
    参阅全部 Ephrin A2 一抗
  • 描述
    兔多克隆抗体to Ephrin A2
  • 特异性ab72661 detects endogenous levels of fragment of activated Ephrin A2 resulting from cleavage adjacent to Asn188.
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide derived from human Ephrin A2

  • 阳性对照
    • Extracts from 293 cells treated with etoposide (25uM, 1hour).

性能

应用

Our Abpromise guarantee covers the use of ab72661 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 21 kDa (predicted molecular weight: 24 kDa).
ELISA 1/20000.

靶标

Anti-Ephrin A2 antibody 图像

  • All lanes : Anti-Ephrin A2 antibody (ab72661) at 1/500 dilution

    Lane 1 : Extracts from 293 cells, treated with etoposide (25uM, 1hour)
    Lane 2 : Extracts from 293 cells, treated with etoposide (25uM, 1hour) with Cl-peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 24 kDa
    Observed band size : 21 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 85 kDa. We are unsure as to the identity of these extra bands.

Anti-Ephrin A2 antibody (ab72661)参考文献

ab72661 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (many normal and tumor cell lines)
Specification many normal and tumor cell lines
Permeabilization No
Username

Abcam user community

Verified customer

提交于 Jul 22 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"