Anti-Eph receptor B4/HTK抗体[RM0056-6A14] (ab73259)
Key features and details
- Rat monoclonal [RM0056-6A14] to Eph receptor B4/HTK
- Suitable for: IHC-P
- Reacts with: Mouse
- Isotype: IgG1
概述
-
产品名称
Anti-Eph receptor B4/HTK抗体[RM0056-6A14]
参阅全部 Eph receptor B4/HTK 一抗 -
描述
大鼠单克隆抗体[RM0056-6A14] to Eph receptor B4/HTK -
宿主
Rat -
特异性
ab73259 is specific for mouse Eph receptor B4/HTK. It does not cross-react with recombinant mouse EphA2, EphA3, EphA4, EphA6, EphA7 and EphB6
-
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Mouse -
免疫原
Recombinant fragment corresponding to Mouse Eph receptor B4/HTK (extracellular).
Database link: P54761 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Constituent: PBS -
Concentration information loading...
-
纯度
Protein A purified -
纯化说明
ab73259 was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat immunized with recombinant mouse Eph receptor B4/HTK extracellular domain). -
克隆
单克隆 -
克隆编号
RM0056-6A14 -
同种型
IgG1 -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab73259于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P | (1) |
1/100 - 1/200.
Perform enzymatic antigen retrieval with 20 ug/ml proteinase K before commencing with IHC protocol. |
说明 |
---|
IHC-P
1/100 - 1/200. Perform enzymatic antigen retrieval with 20 ug/ml proteinase K before commencing with IHC protocol. |
靶标
-
功能
Receptor for members of the ephrin-B family. Binds to ephrin-B2. May have a role in events mediating differentiation and development. -
组织特异性
Abundantly expressed in placenta and in a range of primary tissues and malignant cell lines. Expressed in fetal, but not adult, brain, and in primitive and myeloid, but not lymphoid, hematopoietic cells. -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
Contains 2 fibronectin type-III domains.
Contains 1 protein kinase domain.
Contains 1 SAM (sterile alpha motif) domain. -
翻译后修饰
Autophosphorylated. -
细胞定位
Membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 13846 Mouse
- SwissProt: P54761 Mouse
- Unigene: 34533 Mouse
-
别名
- Ephb4 antibody
- EPHB4_HUMAN antibody
- Ephrin receptor EphB4 antibody
see all
图片
-
ab73259 at 1/200 dilution, staining Eph receptor B4/HTK in LPS treated mouse kidney tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections).
-
ab73259 staining the Eph receptor B4/HTK in Mouse lymph node tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 1 hour at 20°C; antigen retrieval was not performed. Samples were incubated with primary antibody (1/100) for 12 hours at 4°C. A Biotin-conjugated Rabbit anti-rat polyclonal (1/200) was used as the secondary antibody.
数据表及文件
-
Datasheet download
文献 (4)
ab73259 被引用在 4 文献中.
- Wang Y et al. Loading of erythropoietin on biphasic calcium phosphate bioceramics promotes osteogenesis and angiogenesis by regulating EphB4/EphrinB2 molecules. J Mater Sci Mater Med 33:19 (2022). PubMed: 35072831
- Sagar V et al. EPHB4 inhibition activates ER stress to promote immunogenic cell death of prostate cancer cells. Cell Death Dis 10:801 (2019). PubMed: 31641103
- Munro DAD et al. Refuting the hypothesis that semaphorin-3f/neuropilin-2 exclude blood vessels from the cap mesenchyme in the developing kidney. Dev Dyn 246:1047-1056 (2017). PubMed: 28929539
- Chen II et al. EphrinB2 regulates the emergence of a hemogenic endothelium from the aorta. Sci Rep 6:27195 (2016). ICC/IF ; Mouse . PubMed: 27250641