Anti-Eph receptor B4抗体(ab93902)


  • 产品名称Anti-Eph receptor B4抗体
    参阅全部 Eph receptor B4 一抗
  • 描述
    兔多克隆抗体to Eph receptor B4
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Cow, Monkey
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 850 - 950 of Human Eph receptor B4.

  • 阳性对照
    • This antibody gave a positive signal in both Human placenta tissue lysate and A549 whole cell lysate.



Our Abpromise guarantee covers the use of ab93902 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 125 kDa (predicted molecular weight: 108 kDa).

Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product.


  • 功能Receptor for members of the ephrin-B family. Binds to ephrin-B2. May have a role in events mediating differentiation and development.
  • 组织特异性Abundantly expressed in placenta and in a range of primary tissues and malignant cell lines. Expressed in fetal, but not adult, brain, and in primitive and myeloid, but not lymphoid, hematopoietic cells.
  • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 1 protein kinase domain.
    Contains 1 SAM (sterile alpha motif) domain.
  • 翻译后修饰Autophosphorylated.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Ephb4 antibody
    • EPHB4_HUMAN antibody
    • Ephrin receptor EphB4 antibody
    • Ephrin type-B receptor 4 antibody
    • Hepatoma Transmembrane Kinase antibody
    • HTK antibody
    • MYK 1 antibody
    • MYK1 antibody
    • TYRO 11 antibody
    • TYRO11 antibody
    • Tyrosine-protein kinase receptor HTK antibody
    • Tyrosine-protein kinase TYRO11 antibody
    see all

Anti-Eph receptor B4 antibody 图像

  • All lanes : Anti-Eph receptor B4 antibody (ab93902) at 1 µg/ml

    Lane 1 : Human placenta tissue lysate - total protein (ab29745)
    Lane 2 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 108 kDa
    Observed band size : 125 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 26 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

    The predicted molecular weight of Eph receptor B4 is 108 kDa (SwissProt), however we expect to observe a banding pattern at 125 kDa. Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

Anti-Eph receptor B4 antibody (ab93902)参考文献

ab93902 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 50000 cells
Gel Running Conditions Reduced Denaturing (10% gel)
Sample Human Cell lysate - whole cell (A549, HEK293)
Specification A549, HEK293
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

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提交于 Apr 02 2014