Anti-Eph receptor B4抗体(ab10618)


  • 产品名称Anti-Eph receptor B4抗体
    参阅全部 Eph receptor B4 一抗
  • 描述
    山羊多克隆抗体to Eph receptor B4
  • 经测试应用适用于: ELISA, Dot blot, Inhibition Assaymore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Purified recombinant mouse EphB4 extracellular domain expressed in mouse NSO cells.



Our Abpromise guarantee covers the use of ab10618 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Dot blot
Inhibition Assay
  • 应用说明Dot: Use at a concentration of 0.1 - 0.2 µg/ml. The detection limit for recombinant mouse EphB4 is approximately 5 ng/lane under non-reducing and reducing conditions.
    ELISA: Use at a concentration of 0.5 - 1.0 µg/ml, detection limit 1.5 ng/well.
    Inhib: Use at a concentration of 0.5 - 2 µg/ml. For neutralization of receptor-ligand interaction, approximately 0.5-2 µg/ml of the antibody will block 50% of the binding of recombinant mouse ephrin-B2/ (5 ng/ml) to immobilized recombinant mouse EphB4/Fc chimera (100 µl of a 2 µg/ml solution coated in each well).

    The recombinant mouse EphB4 used for the testing of this antibody consists of the extracellular domain of mouse EphB4 (amino acids 1-539) fused by means of a polypeptide linker to the Fc portion of human IgG1 that is histidine-tagged at the C-terminus. N-terminal sequencing indicates that recombinant mouse EphB4/Fc has Leu 16 at the amino terminus. The calculated molecular mass of the reduced protein is approximately 85.2 kDa, but as a result of glycosylation, recombinant EphB4/Fc migrates as an approximately 110 kDa protein under reducing conditions in SDS-PAGE. EphB4 binds to ephrin-B1 and ephrin-B2. Human and mouse EphB4 extracellular domains share approximately 88% homology.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Receptor for members of the ephrin-B family. Binds to ephrin-B2. May have a role in events mediating differentiation and development.
    • 组织特异性Abundantly expressed in placenta and in a range of primary tissues and malignant cell lines. Expressed in fetal, but not adult, brain, and in primitive and myeloid, but not lymphoid, hematopoietic cells.
    • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
      Contains 2 fibronectin type-III domains.
      Contains 1 protein kinase domain.
      Contains 1 SAM (sterile alpha motif) domain.
    • 翻译后修饰Autophosphorylated.
    • 细胞定位Membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Ephb4 antibody
      • EPHB4_HUMAN antibody
      • Ephrin receptor EphB4 antibody
      • Ephrin type-B receptor 4 antibody
      • Hepatoma Transmembrane Kinase antibody
      • HTK antibody
      • MYK 1 antibody
      • MYK1 antibody
      • TYRO 11 antibody
      • TYRO11 antibody
      • Tyrosine-protein kinase receptor HTK antibody
      • Tyrosine-protein kinase TYRO11 antibody
      see all

    Anti-Eph receptor B4 antibody (ab10618)参考文献

    ab10618 has not yet been referenced specifically in any publications.

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