Anti-Eph receptor B1 + Eph receptor B2 (phospho Y594)抗体(ab61791)

概述

  • 产品名称Anti-Eph receptor B1 + Eph receptor B2 (phospho Y594)抗体
    参阅全部 Eph receptor B1 + Eph receptor B2 一抗
  • 描述
    兔多克隆抗体to Eph receptor B1 + Eph receptor B2 (phospho Y594)
  • 特异性ab61791 detects endogenous levels of EPHB1 only when phosphorylated at tyrosine 594.
  • 经测试应用适用于: WB, ICC/IF, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthesized phosphopeptide derived from human EPHB1 around the phosphorylation site of tyrosine 594 (K-I-YP-I-D).

  • 阳性对照
    • Extracts from HepG2 cells, HuvEc cells.

性能

应用

Our Abpromise guarantee covers the use of ab61791 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).
ICC/IF 1/500 - 1/1000.
ELISA 1/5000.

靶标

  • 相关性Ephrin receptors bind members of the ephrin ligand family. They are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. They make up the largest subgroup of the receptor tyrosine kinase (RTK) family. Eph receptor B1 (EphB1) and Eph receptor B2 (EphB2) are receptors for ephrin-B family members. Developing and adult neural tissue express nearly all of the Ephrin receptors and ephrin ligands. Ephrins and ephrin receptors also play a significant role in angiogenesis. EphB2 acts as a tumor suppressor.
  • 细胞定位Membrane; Single-pass type I membrane protein.
  • 数据库链接
  • 别名
    • EPH B1 antibody
    • EPH B2 antibody
    • Eph tyrosine kinase 2 antibody
    • Eph tyrosine kinase 3 antibody
    • EPHB1 antibody
    • EPHB2 antibody
    • Ephrin receptor EphB1 antibody
    • Ephrin receptor EphB2 antibody
    • Ephrin type B receptor 1 antibody
    • Ephrin type B receptor 2 antibody
    • EPHT 2 antibody
    • EPHT 3 antibody
    • ERK antibody
    • Hek 5 antibody
    • Hek 6 antibody
    • Tyrosine protein kinase receptor EPH2 antibody
    • Tyrosine protein kinase receptor EPH3 antibody
    see all

Anti-Eph receptor B1 + Eph receptor B2 (phospho Y594) antibody 图像

  • All lanes : Anti-Eph receptor B1 + Eph receptor B2 (phospho Y594) antibody (ab61791) at 1/1000 dilution

    Lane 1 : HeLa whole cell lysate
    Lane 2 : HeLa whole cell lysate - overexpressing EphB2
    Lane 3 : HeLa whole cell lysate - overexpressinf EphB2-kinase dead (K661R)

    Lysates/proteins at 100000 cells per lane.

    Secondary
    HRP-conjugated goat anti-rabbit IgG monoclonal at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 110 kDa
    Observed band size : 110 kDa


    Exposure time : 5 minutes

    This image is courtesy of an Abreview submitted by Thomas Jungas

    All cells were stimulated with preclustered ephrinb1-Fc chimeric protein.

    See Abreview

  • ab61791 staining Eph receptor B1 + Eph receptor B2 (phospho Y594) in HeLa cells (overexpressing  EphB2 and stimulated for 20 minutes with preclustered efnb1-Fc chimeric protein) by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 1% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/100 in PBS + 1% BSA + 0.1% Triton X-100) for 2 hour at 25°C. An Alexa Fluor® 488-conjugated donkey anti-rabbit IgG polyclonal (1/300) was used as the secondary antibody. Red - anti-EphB2 (goat), Grey - DAPI, Green - ab61791.

    See Abreview

  • All lanes : Anti-Eph receptor B1 + Eph receptor B2 (phospho Y594) antibody (ab61791) at 1/500 dilution

    Lane 1 : Extracts from HepG2 cells
    Lane 2 : Extracts from HepG2 cells with the immunising phosphopeptide


    Predicted band size : 110 kDa
    Observed band size : 110 kDa
  • Immunofluorescence analysis of HuvEc cells, using ab61791 at 1/500-1/1000 dilution with (+) or without (-) immunising phosphopeptide.

Anti-Eph receptor B1 + Eph receptor B2 (phospho Y594) antibody (ab61791)参考文献

This product has been referenced in:
  • Jungas T  et al. Eph-mediated tyrosine phosphorylation of citron kinase controls abscission. J Cell Biol 214:555-69 (2016). WB, ICC/IF . Read more (PubMed: 27551053) »
  • Wilson C  et al. Overcoming EMT-associated resistance to anti-cancer drugs via Src/FAK pathway inhibition. Oncotarget 5:7328-41 (2014). WB ; Human . Read more (PubMed: 25193862) »

See all 2 Publications for this product

Product Wall

Application Western blot
Loading amount 100000 cells
Gel Running Conditions Reduced Denaturing (4-20%)
Sample Human Cell lysate - whole cell (HeLa cells)
Specification HeLa cells
Treatment stimulated with 1´g/ml clustered ephrinb1-Fc
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mr. Thomas Jungas

Verified customer

提交于 Apr 11 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Sample Human Cell (HeLa cells)
Specification HeLa cells
Permeabilization Yes - Triton X-100 @ 0.1%
Fixative Paraformaldehyde
Username

Mr. Thomas Jungas

Verified customer

提交于 Mar 21 2014

ab61791 detects Eph B1 receptor phosphorylation at Y594 and the corresponding phosphorylation status of Eph B2 receptor at Y596.

In terms of the significance of Eph B2 receptor phosphorylation at Y596, a literature search through some of the r...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"