Anti-Eph receptor B1 + Eph receptor B2 + Eph receptor B3 + Eph receptor B4抗体(ab64820)

概述

  • 产品名称Anti-Eph receptor B1 + Eph receptor B2 + Eph receptor B3 + Eph receptor B4抗体
    参阅全部 Eph receptor B1 + Eph receptor B2 + Eph receptor B3 + Eph receptor B4 一抗
  • 描述
    兔多克隆抗体to Eph receptor B1 + Eph receptor B2 + Eph receptor B3 + Eph receptor B4
  • 特异性ab64820 detects endogenous levels of total Eph receptor B4 protein. We have not performed cross-reactivity testing to other class B Eph receptors. The immunogen has high homology to EPHB1(100%), EPHB3 (100%), EPHB2 (92%).
  • 经测试应用适用于: IHC-P, ELISA, WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide derived from internal region of human Eph receptor B4.

  • 阳性对照
    • 293 and Jurkat cell extracts.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab64820 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ELISA 1/20000.
WB 1/500 - 1/1000. Detects a band of approximately 108 kDa (predicted molecular weight: 108 kDa).

靶标

  • 功能Receptor for members of the ephrin-B family. Binds to ephrin-B1, -B2 and -B3. Binding with the guidance cue ephrin-B2 at the optic chiasm midline redirect ventrotemporal (VT) retinal ganglion cells (RGCs) axons ipsilaterally. May be involved in cell-cell interactions in the nervous system.
  • 组织特异性Preferentially expressed in brain.
  • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 1 protein kinase domain.
    Contains 1 SAM (sterile alpha motif) domain.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CAPB antibody
    • DRT antibody
    • EK2 antibody
    • EK5 antibody
    • EK6 antibody
    • ELK antibody
    • elk related tyrosine kinase antibody
    • EPH like kinase 2 antibody
    • EPH like kinase 6 antibody
    • EPH like tyrosine kinase 2 antibody
    • eph tyrosine kinase 2 antibody
    • eph tyrosine kinase 3 antibody
    • EPH-like kinase 6 antibody
    • Ephb1 antibody
    • EPHB1_HUMAN antibody
    • EPHB2 antibody
    • EPHB3 antibody
    • EPHB4 antibody
    • ephrin receptor EphB4 antibody
    • ephrin type B receptor 1 antibody
    • ephrin type B receptor 2 antibody
    • ephrin type B receptor 3 antibody
    • ephrin type B receptor 4 antibody
    • Ephrin type-B receptor 1 antibody
    • EPHT2 antibody
    • EPHT3 antibody
    • ERK antibody
    • ETK2 antibody
    • Hek 5 antibody
    • Hek 6 antibody
    • HEK2 antibody
    • HEK5 antibody
    • HEK6 antibody
    • hepatoma transmembrane kinase antibody
    • HTK antibody
    • human embryo kinase 2 antibody
    • MYK1 antibody
    • NET antibody
    • PCBC antibody
    • protein tyrosine kinase HEK5 antibody
    • renal carcinoma antigen NY REN 47 antibody
    • soluble EPHB1 variant 1 antibody
    • soluble EPHB4 variant 1 antibody
    • soluble EPHB4 variant 2 antibody
    • soluble EPHB4 variant 3 antibody
    • TYRO11 antibody
    • Tyro5 antibody
    • TYRO6 antibody
    • tyrosine protein kinase receptor EPH 2 antibody
    • tyrosine protein kinase receptor EPH 3 antibody
    • tyrosine protein kinase receptor EPH2 antibody
    • tyrosine protein kinase receptor EPH3 antibody
    • tyrosine protein kinase receptor HTK antibody
    • tyrosine protein kinase TYRO11 antibody
    • tyrosine protein kinase TYRO5 antibody
    • tyrosine protein kinase TYRO6 antibody
    • Tyrosine-protein kinase receptor EPH-2 antibody
    see all

Anti-Eph receptor B1 + Eph receptor B2 + Eph receptor B3 + Eph receptor B4 antibody 图像

  • All lanes : Anti-Eph receptor B1 + Eph receptor B2 + Eph receptor B3 + Eph receptor B4 antibody (ab64820) at 1/500 dilution

    Lane 1 : 293 cell extract at 30 µg
    Lane 2 : Jurkat cell extract at 30 µg
    Lane 3 : immunising peptide at 10 µg


    Predicted band size : 108 kDa
    Observed band size : 108 kDa
  • IHC image of ab64820 staining in human normal lymphoid formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab64820, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Eph receptor B1 + Eph receptor B2 + Eph receptor B3 + Eph receptor B4 antibody (ab64820)参考文献

This product has been referenced in:
  • Model LS  et al. Arterial shear stress reduces eph-b4 expression in adult human veins. Yale J Biol Med 87:359-71 (2014). IHC-P ; Human . Read more (PubMed: 25191151) »
  • Doleman JF  et al. Identification of the Eph receptor pathway as a novel target for eicosapentaenoic acid (EPA) modification of gene expression in human colon adenocarcinoma cells (HT-29). Nutr Metab (Lond) 7:56 (2010). WB ; Human . Read more (PubMed: 20624275) »

See all 2 Publications for this product

Product Wall

Application Western blot
Loading amount 50000 cells
Gel Running Conditions Reduced Denaturing (10% gel)
Sample Human Cell lysate - whole cell (A549, HEK293)
Specification A549, HEK293
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Mar 28 2014

We haven't tested this antibody for cross-reactivity with other class B Eph receptors, but I've aligned the immunogen against the human proteome and found that it is 100% identical to EPHB3, 93% to EPHB1, and 92% to EPHB2. Since these proteins are so h...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (lung tissue containing a vein)
Specification lung tissue containing a vein
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: dako low pH
Permeabilization No
Blocking step HP as blocking agent for 30 minute(s) · Concentration: 2%
Username

Dr. mark southwood

Verified customer

提交于 Oct 27 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"