Anti-Eph receptor A4抗体(ab5396)

概述

  • 产品名称Anti-Eph receptor A4抗体
    参阅全部 Eph receptor A4 一抗
  • 描述
    兔多克隆抗体to Eph receptor A4
  • 经测试应用适用于: ICC/IF, ELISA, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human Eph receptor A4 aa 875-904 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).

  • 阳性对照
    • NCI-H460 cell lysate
  • 常规说明

    Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度Protein G purified
  • 纯化说明This antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
  • Primary antibody说明Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab5396 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/500.
ELISA 1/1000.
WB 1/1000. Detects a band of approximately 108 kDa (predicted molecular weight: 118 kDa).

We recommend using CHAPS lysis buffer rather than RIPA, Invitrogen NuPage gel system, blotting on PVDF membrane, blocking 30' in 10% Fetal Bovine Serum, 3% BSA, 0.3% Tween 20 in 1X PBS - please see Abreview for further information (published on 24th July, 2006).

IHC-P 1/25 - 1/100.

靶标

  • 功能Receptor for members of the ephrin-A family. Binds to ephrin-A1, -A4 and -A5. Binds more poorly to ephrin-A2 and -A3. May play a role in a signal transduction process involved in hindbrain pattern formation.
  • 组织特异性Ubiquitous.
  • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 1 protein kinase domain.
    Contains 1 SAM (sterile alpha motif) domain.
  • 结构域The protein kinase domain mediates interaction with NGEF/ephexin-1.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cek 8 antibody
    • CEK8 antibody
    • EK8 antibody
    • eph receptor a4 antibody
    • EPH-like kinase 8 antibody
    • EPHA4 antibody
    • EPHA4_HUMAN antibody
    • Ephrin type-A receptor 4 antibody
    • HEK 8 antibody
    • hEK8 antibody
    • Receptor protein-tyrosine kinase HEK8 antibody
    • Sek 1 antibody
    • SEK antibody
    • TYRO 1 protein tyrosine kinase antibody
    • TYRO1 antibody
    • Tyrosine-protein kinase receptor SEK antibody
    • Tyrosine-protein kinase TYRO1 antibody
    see all

Anti-Eph receptor A4 antibody 图像

  • All lanes : Anti-Eph receptor A4 antibody (ab5396) at 1/1000 dilution

    Lane 1 : HeLa Cell Lysate
    Lane 2 : NCI-H460 Cell Lysate
    Lane 3 : Mouse NIH3T3 Cell Lysate
    Lane 4 : Human ovary tissue Lysate

    Lysates/proteins at 35 µg per lane.

    Secondary
    Goat anti-rabbit IgG H+L HRP conjugated at 1/10000 dilution

    Predicted band size : 118 kDa

    Incubation time was overnight at 4°C. Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-Eph receptor A4 antibody (ab5396) at 1/1000 dilution + NCI-H460 whole cell lysate at 35 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution

    Predicted band size : 118 kDa

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • ab5396 at 1/500 staining rat cerebellar granual cells by ICC/IF. The cells were permeabilized with 0.1% Triton X-100, paraformaldehyde fixed and blocked with 2% serum prior to incubation with the antibody for 1 hour. A Cy3 conjugated goat anti-rabbit was used as the secondary.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labeling Eph receptor A4 with ab5396 at dilution 1/25. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at 37°C; heat mediated antigen retrieval was performed using a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hour at 37°C. A Peroxidase-conjugated Goat anti-rabbit polyclonal (ready to use) was used as the secondary antibody.

Anti-Eph receptor A4 antibody (ab5396)参考文献

ab5396 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application IHC - Wholemount
Sample Marmoset (common) Tissue (Adult Brain)
Specification Adult Brain
Username

煜欽 林

Verified customer

提交于 Jun 05 2013

The new lot has been tested in WB so it will be OK.

I have requested image from laboratory, which I forward to you soon.

Thank you very much for having patience.

We have now acquired new lot of this antibody. I will inform you soon with the shipment date.

Please let me know if you have any question.

Thank you for your email.

I have been in contact with laboratory for acquiring new lot. I will send you a confirmation once we ship it.

Many thanks for having patience. Please do not hesitate to contact me if you have any question.

Dr. Tanya is on holiday until 2nd January so I am dealing with her inquires.

Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results.

I appr...

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Thank you for your enquiry regarding ab5396 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody.

Though you have kindly provided some details, it w...

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I am sorry to hear that you have been experiencing problems using this product in the application that you wish.

I can confirm that ab5396 has been tested for both Western blot and for Immunohistochemistry.

In order to assess the qu...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Brain C57Bl/6 mice)
Loading amount 30 µg
Specification Brain C57Bl/6 mice
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3%
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Abcam user community

Verified customer

提交于 Aug 23 2006

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (P0 RAT Spinal Cord)
Loading amount 30 µg
Specification P0 RAT Spinal Cord
Blocking step Other as blocking agent for 30 minute(s) · Concentration: 3%BSA, 10%
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Abcam user community

Verified customer

提交于 Aug 14 2006

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Cerebellar granule cells)
Specification Cerebellar granule cells
Fixative Paraformaldehyde
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 2% goat se
Username

Abcam user community

Verified customer

提交于 Aug 09 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"