Anti-Eph receptor A4抗体(ab5389)

概述

  • 产品名称Anti-Eph receptor A4抗体
    参阅全部 Eph receptor A4 一抗
  • 描述
    兔多克隆抗体to Eph receptor A4
  • 经测试应用适用于: IHC-P, ICC/IF, IHC-FrFl, Flow Cyt, WBmore details
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human Eph receptor A4 aa 40-70 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).

  • 阳性对照
    • WB: HeLa whole cell lysate. IHC-P: Human breast carcinoma tissue. IHC-FrFl: Rat cortex tissue. ICC/IF: Human stem cells. FC: CEM cells.

性能

应用

Our Abpromise guarantee covers the use of ab5389 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/100.
ICC/IF 1/100.
IHC-FrFl 1/100 - 1/300.
Flow Cyt 1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
WB 1/1000. Detects a band of approximately 108 kDa (predicted molecular weight: 118 kDa).

靶标

  • 功能Receptor for members of the ephrin-A family. Binds to ephrin-A1, -A4 and -A5. Binds more poorly to ephrin-A2 and -A3. May play a role in a signal transduction process involved in hindbrain pattern formation.
  • 组织特异性Ubiquitous.
  • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 1 protein kinase domain.
    Contains 1 SAM (sterile alpha motif) domain.
  • 结构域The protein kinase domain mediates interaction with NGEF/ephexin-1.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cek 8 antibody
    • CEK8 antibody
    • EK8 antibody
    • eph receptor a4 antibody
    • EPH-like kinase 8 antibody
    • EPHA4 antibody
    • EPHA4_HUMAN antibody
    • Ephrin type-A receptor 4 antibody
    • HEK 8 antibody
    • hEK8 antibody
    • Receptor protein-tyrosine kinase HEK8 antibody
    • Sek 1 antibody
    • SEK antibody
    • TYRO 1 protein tyrosine kinase antibody
    • TYRO1 antibody
    • Tyrosine-protein kinase receptor SEK antibody
    • Tyrosine-protein kinase TYRO1 antibody
    see all

Anti-Eph receptor A4 antibody 图像

  • All lanes : Anti-Eph receptor A4 antibody (ab5389) at 1/1000 dilution

    Lane 1 : 293 cell lysate - nontransfected
    Lane 2 : 293 cell lysate - transfected with EphA4

    Lysates/proteins at 2 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

    Predicted band size : 118 kDa

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunofluorescent staining of methanol/acetone fixed human stem cells for Eph4A (blue) and endothelial Lectin(red). Data kindly provided by Dr. Weis from Cheresh Lab, UCSD.
  • ab5389 EphA4 antibody immunostaining cortical neurons (image taken at X2; cytoplasmic staining).Bar=20 microns.

    Protocol: ab5389 incubated overnight at room temperature at a dilution of 1/100-1/300 on free floating perfusion fixed coronal sections of brain. Immunostaining visualised by direct fluorescence (Alexa Fluor® 488, 1/1000).

  • ab5389 EHpA4 antibody immunostaining hippocampal neurons ( X2 magnification; cytoplasmic staining). Bar: 20 microns.

    Protocol: ab5389 incubated overnight at room temperature at a dilution of 1/100-1/300 on free floating perfusion fixed coronal sections of brain. Immunostaining visualised by direct fluorescence (Alexa Fluor® 488, 1/1000).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (BC) tissue labeling Eph receptor A4 with ab5389 at 1/25. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at 37°C. Heat mediated antigen retrieval was performed using a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hour at 37°C. A Peroxidase-conjugated Goat anti-rabbit polyclonal (ready to use) was used as the secondary antibody.

  • Anti-Eph receptor A4 antibody (ab5389) at 1/100 dilution + HeLa cell lysate

    Secondary
    HRP-conjugated anti-rabbit IgG

    Predicted band size : 118 kDa
  • All lanes : Anti-Eph receptor A4 antibody (ab5389) at 1/1000 dilution

    Lane 1 : 293 cell lysate - nontransfected
    Lane 2 : 293 cell lysate - transfected with EphA4

    Lysates/proteins at 2 µg per lane.


    Predicted band size : 118 kDa
  • Flow cytometry analysis of CEM cells labelling Eph receptor A4 (green) with ab5389 compared to a negative control (blue). The cells were fixed with paraformaldehyde and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 3% BSA to block non-specific protein-protein interactions followed by the antibody (ab5389, 1µg/1x106 cells) for 60 min at 37ºC. The secondary antibody used was FITC conjugated Goat anti-Rabbit antibodies at 1/200 dilution for 40 min at room temperature. Blank control (blue line) was used under the same conditions. Acquisition of >10,000 events was performed.

Anti-Eph receptor A4 antibody (ab5389)参考文献

ab5389 has not yet been referenced specifically in any publications.

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