Anti-Eph receptor A3抗体(ab54623)


  • 产品名称Anti-Eph receptor A3抗体
    参阅全部 Eph receptor A3 一抗
  • 描述
    小鼠单克隆抗体to Eph receptor A3
  • 经测试应用适用于: ICC/IF, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 202-326 of Human Eph receptor A3



Our Abpromise guarantee covers the use of ab54623 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 110 kDa.
IHC-P Use a concentration of 5 µg/ml.


  • 功能Receptor for members of the ephrin-A family. Binds to ephrin-A2, -A3, -A4 and -A5. Could play a role in lymphoid function.
  • 组织特异性Widely expressed. Highest level in placenta.
  • 疾病相关Defects in EPHA3 may be a cause of colorectal cancer (CRC) [MIM:114500].
  • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 1 protein kinase domain.
    Contains 1 SAM (sterile alpha motif) domain.
  • 细胞定位Secreted and Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AW492086 antibody
    • Cek4 antibody
    • EC antibody
    • EK4 antibody
    • End3 antibody
    • Eph receptor A3 antibody
    • EPH-like kinase 4 antibody
    • EPH-like tyrosine kinase 1 antibody
    • EPHA3 antibody
    • EPHA3_HUMAN antibody
    • Ephrin receptor EphA3 antibody
    • Ephrin type-A receptor 3 antibody
    • ETK 1 antibody
    • ETK antibody
    • ETK1 antibody
    • HEK 4 antibody
    • HEK antibody
    • HEK4 antibody
    • Human embryo kinase 1 antibody
    • Human embryo kinase antibody
    • Mek4 antibody
    • MGC109882 antibody
    • Receptor tyrosine kinase HEK antibody
    • Tyro 4 antibody
    • Tyro4 antibody
    • TYRO4 protein tyrosine kinase antibody
    • Tyrosine protein kinase receptor ETK 1 antibody
    • Tyrosine-protein kinase receptor ETK1 antibody
    • Tyrosine-protein kinase TYRO4 antibody
    see all

Anti-Eph receptor A3 antibody 图像

  • Predicted band size : 110 kDa
    Eph receptor A3 antibody (ab54623) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • ICC/IF image of ab54623 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab54623, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab54623 staining in Human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab54623, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Eph receptor A3 antibody (ab54623)参考文献

ab54623 has not yet been referenced specifically in any publications.

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I am very pleased to hear the customer would like make use of ourtesting discountoffer and test ab54623 Anti-Eph receptor A3 antibody in flow cytometry.

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Thank you for contacting us.
You are right; the immunogen is indeed present in the extracellular domain of the protein so there is no need for permeabilization. You may skip this step.
We unfortunately do not have any additional data for shar...

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