Anti-Eph receptor A2+A3+A4 (phospho Y588 + Y596)抗体(ab62256)

概述

  • 产品名称Anti-Eph receptor A2+A3+A4 (phospho Y588 + Y596)抗体
    参阅全部 Eph receptor A2+A3+A4 一抗
  • 描述
    兔多克隆抗体to Eph receptor A2+A3+A4 (phospho Y588 + Y596)
  • 特异性ab62256 detects endogenous levels of Eph receptor A2+A3+A4 only when phosphorylated at tyrosine 588/596.
  • 经测试应用适用于: ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Human, Sea Urchin
    预测可用于: Mouse
  • 免疫原

    Synthetic phosphopeptide derived from human Eph receptor A2+A3+A4 around the phosphorylation site of tyrosine 588/596 (T-YP-V-D-P).

  • 阳性对照
    • HeLa cells.

性能

应用

Our Abpromise guarantee covers the use of ab62256 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/20000.
ICC/IF 1/500 - 1/1000.

靶标

Anti-Eph receptor A2+A3+A4 (phospho Y588 + Y596) antibody 图像

  • ab62256 at 1/500 - 1/1000 dilution staining Eph receptor A2+A3+A4 in HeLa cells by Immunofluorescence, in the absence or presence of the immunising peptide.

Anti-Eph receptor A2+A3+A4 (phospho Y588 + Y596) antibody (ab62256)参考文献

This product has been referenced in:
  • Sharonov GV  et al. Point mutations in dimerization motifs of the transmembrane domain stabilize active or inactive state of the EphA2 receptor tyrosine kinase. J Biol Chem 289:14955-64 (2014). ICC/IF ; Human . Read more (PubMed: 24733396) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Sea Urchin Cell (Embryo, Strongylocentrotus purpuratus)
Specification Embryo, Strongylocentrotus purpuratus
Fixative Paraformaldehyde
Permeabilization Yes - Superblock with 0.03% TritonX-100, 1 mM Na3VO4, 1 mM NaF
Blocking step (agent) for 30 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Dr. Robert Burke

Verified customer

提交于 Mar 28 2013

Thank you for contacting us.

The lab sent me more information about the immunogen of the antibody ab62256:

The antibody is against both EPHA2 and EPHA3. This antibody recognizes EPHA2 when it is phosphorylated at Y588 and EPHA3 when i...

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Thank you for contacting us.

The immunogen for the anti-phosphorylated Eph receptor antibody, ab62256, is identical to the sequence you provided, at least around Y588. I have asked the laboratory to provide a more precise alignment (I do not...

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Thank you for contacting us. I am sorry to hear that this antibody did not work as expected. I can certainly arrange a refund for you, but would like to gather some more information about the actual problem for our records. Please let me know: ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"