Anti-Eph receptor A2抗体[3D7] (ab118882)

概述

  • 产品名称
    Anti-Eph receptor A2抗体[3D7]
    参阅全部 Eph receptor A2 一抗
  • 描述
    小鼠单克隆抗体[3D7] to Eph receptor A2
  • 经测试应用
    适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 559-976 of Human Eph receptor A2 (NP_004422).

  • 阳性对照
    • WB: Mouse brain extracts IHC-P: Human testis, thyroid, colon cancer and gastric cancer tissues.

性能

应用

Our Abpromise guarantee covers the use of ab118882 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/2000. Predicted molecular weight: 108 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能
    Receptor for members of the ephrin-A family. Binds to ephrin-A1, -A3, -A4 and -A5. Plays an important role in angiogenesis and tumor neovascularization. The recruitement of VAV2, VAV3 and PI3-kinase p85 subunit by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly (By similarity). Induces apoptosis in a p53/TP53-independent, caspase-8-dependent manner.
  • 组织特异性
    Expressed in brain and glioma tissue and glioma cell lines (at protein level). Expressed most highly in tissues that contain a high proportion of epithelial cells, e.g., skin, intestine, lung, and ovary.
  • 疾病相关
    Genetic variations in EPHA2 are the cause of susceptibility to cataract cortical age-related type 2 (ARCC2) [MIM:613020]. A developmental punctate opacity common in the cortex and present in most lenses. The cataract is white or cerulean, increases in number with age, but rarely affects vision.
    Defects in EPHA2 are the cause of cataract posterior polar type 1 (CTPP1) [MIM:116600]. A subcapsular opacity, usually disk-shaped, located at the back of the lens. It can have a marked effect on visual acuity.
  • 序列相似性
    Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 1 protein kinase domain.
    Contains 1 SAM (sterile alpha motif) domain.
  • 翻译后修饰
    Activated by EFNA1 via tyrosine phosphorylation. Phosphorylated residues Tyr-588 and Tyr-594 are required for binding VAV2 and VAV3 while phosphorylated residues Tyr-735 and Tyr-930 are required for binding PI3-kinase p85 subunit. These phosphorylated residues are critical for recruitment of VAV2 and VAV3 and PI3-kinase p85 subunit which transduce downstream signaling to activate RAC1 GTPase and endothelial cell migration. They also play a critical role in transducing EPHA2 signaling in vascular endothelial cells during tumor angiogenesis.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ARCC2 antibody
    • AW545284 antibody
    • CTPA antibody
    • CTPP1 antibody
    • CTRCT6 antibody
    • EC 2.7.10.1 antibody
    • Eck antibody
    • Eph receptor A2 antibody
    • EPHA2 antibody
    • EPHA2_HUMAN antibody
    • Ephrin receptor antibody
    • Ephrin receptor EphA2 antibody
    • Ephrin type A receptor 2 antibody
    • Ephrin type-A receptor 2 antibody
    • Epithelial cell kinase antibody
    • Epithelial cell receptor protein tyrosine kinase antibody
    • Myk 2 antibody
    • Myk2 antibody
    • Sek 2 antibody
    • Sek2 antibody
    • Soluble EPHA2 variant 1 antibody
    • Tyrosine protein kinase receptor ECK antibody
    • Tyrosine-protein kinase receptor ECK antibody
    • Tyrosine-protein kinase receptor MPK-5 antibody
    • Tyrosine-protein kinase receptor SEK-2 antibody
    see all

Anti-Eph receptor A2 antibody [3D7] 图像

  • Anti-Eph receptor A2 antibody [3D7] (ab118882) at 1/500 dilution + Mouse brain extracts at 50 µg

    Secondary
    Goat anti-Mouse conjugated to HRP
    Developed using the ECL technique

    Predicted band size : 108 kDa
  • Human testis (formalin fixed, paraffin embedded) stained with ab118882 at 5 µg/ml, followed by biotinylated anti-Mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Human thyroid tissue (formalin fixed, paraffin embedded) stained with ab118882 at 5 µg/ml, followed by biotinylated anti-Mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Paraffin embedded sections of Human colon cancer tissue were incubated with ab118882 (1/100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1 M sodium citrate buffer and detected using Diaminobenzidine (DAB).
  • Paraffin embedded sections of Human gastric cancer tissue were incubated with ab18882 (1/100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1 M sodium citrate buffer and detected using Diaminobenzidine (DAB).

Anti-Eph receptor A2 antibody [3D7] (ab118882)参考文献

ab118882 has not yet been referenced specifically in any publications.

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (undifferentiated Human ES cells on MEFs)
Permeabilization
Yes - see blocking buffer
Specification
undifferentiated Human ES cells on MEFs
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C
Fixative
Paraformaldehyde
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Verified customer

提交于 Apr 05 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HUES7 ES cells)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
20 µg
Specification
HUES7 ES cells
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
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提交于 Feb 22 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEPG2)
Specification
HEPG2
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C
Fixative
Paraformaldehyde
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提交于 Nov 18 2015

Application
Western blot
Sample
Human Cell lysate - whole cell (HEPG2)
Gel Running Conditions
Reduced Denaturing (12.5)
Loading amount
20 µg
Specification
HEPG2
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
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提交于 Nov 13 2015

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
1% serum + 0.1% BSA in PBS as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 16°C
Sample
Mouse Cell (mouse embyonic stem cells)
Specification
mouse embyonic stem cells
Permeabilization
Yes - 0.1% triton
Fixative
Paraformaldehyde
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提交于 Apr 23 2014

Application
Flow Cytometry
Fixation
Paraformaldehyde
Permeabilization
No
Sample
Mouse Cell (mouse embryonic stem cells)
Specification
mouse embryonic stem cells
Gating Strategy
live cells in fsc vs ssc
Preparation
Cell harvesting/tissue preparation method: cell dissociation buffer
Sample buffer: 0.2% BSA, 0.1% NaAz in PBS
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提交于 Mar 03 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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