概述

  • 产品名称Anti-Eph receptor A1抗体
    参阅全部 Eph receptor A1 一抗
  • 描述
    兔多克隆抗体to Eph receptor A1
  • 经测试应用适用于: Flow Cyt, IHC-P, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to Human Eph receptor A1 aa 29-60 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).

  • 阳性对照
    • WB: HeLa cell lysate. IHC-P: Human hepatocarcinoma tissue. FC: HepG2 cells.

性能

应用

Our Abpromise guarantee covers the use of ab5376 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt 1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P 1/50 - 1/100.
WB 1/1000. Detects a band of approximately 107 kDa (predicted molecular weight: 116 kDa).

靶标

  • 功能Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds with a low affinity EFNA3 and EFNA4 and with a high affinity to EFNA1 which most probably constitutes its cognate/functional ligand. Upon activation by EFNA1 induces cell attachment to the extracellular matrix inhibiting cell spreading and motility through regulation of ILK and downstream RHOA and RAC. Plays also a role in angiogenesis and regulates cell proliferation. May play a role in apoptosis.
  • 组织特异性Overexpressed in several carcinomas.
  • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
    Contains 1 Eph LBD (Eph ligand-binding) domain.
    Contains 2 fibronectin type-III domains.
    Contains 1 protein kinase domain.
    Contains 1 SAM (sterile alpha motif) domain.
  • 翻译后修饰Phosphorylated. Autophosphorylation is stimulated by its ligand EFNA1.
    Ubiquitinated.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • EPH antibody
    • EPH receptor A1 antibody
    • EPH tyrosine kinase 1 antibody
    • EPH tyrosine kinase antibody
    • Epha1 antibody
    • EPHA1_HUMAN antibody
    • Ephrin receptor Eph A1 antibody
    • Ephrin type A receptor 1 antibody
    • Ephrin type-A receptor 1 antibody
    • EPHT 1 antibody
    • EPHT antibody
    • EPHT1 antibody
    • Erythropoietin producing hepatoma amplified sequence antibody
    • Erythropoietin-producing hepatoma receptor antibody
    • Esk antibody
    • hEpha1 antibody
    • MGC163163 antibody
    • Oncogene EPH antibody
    • Soluble EPHA1 variant 1 antibody
    • Soluble EPHA1 variant 2 antibody
    • Tyrosine protein kinase receptor EPH antibody
    • Tyrosine-protein kinase receptor EPH antibody
    see all

Anti-Eph receptor A1 antibody 图像

  • Anti-Eph receptor A1 antibody (ab5376) at 1/100 dilution + HeLa cell lysate

    Predicted band size : 116 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocarcinoma (HC) tissue labelling Eph receptor A1 with ab5376.

  • Flow cytometry analysis of HepG2 cells labelling Eph receptor A1 (green) with ab5376 compared to a negative control (blue). A FITC-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Anti-Eph receptor A1 antibody (ab5376)参考文献

This product has been referenced in:
  • Toma MI  et al. Lack of ephrin receptor A1 is a favorable independent prognostic factor in clear cell renal cell carcinoma. PLoS One 9:e102262 (2014). IHC-P ; Human . Read more (PubMed: 25025847) »

See 1 Publication for this product

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