Anti-EpCAM抗体[HEA125]， prediluted (ab46714)
- 数据表
- 具体的参考文献 (1)
- 实验方案
概述
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产品名称Anti-EpCAM抗体[HEA125]， prediluted
参阅全部 EpCAM 一抗 -
描述小鼠单克隆抗体[HEA125] to EpCAM， prediluted
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经测试应用适用于: ICC/IF, Flow Cyt, IHC-Fr, IHC-Pmore details
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种属反应性与反应: Human
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免疫原
Living human colon carcinoma cell line HT-29
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阳性对照
- Appendix This antibody gave a positive result when used in the following formaldehyde fixed cell lines: MCF-7.
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常规说明
ab46714 reacts intensely with all carcinomas and metastased derived from the intestinal tract, stomach, pancreas, liver, lung, breast, ovary, thyroid gland, kidney, bladder, prostate. Stratified squamous epithelia usually stain less intensely than adenocarcinoma. The antibody is suitable for differentiation between carcinoma and non-carcinoma. Keratinizing areas of a tumour mass usually remain unstained, as do sarcoma, lymphoma, melanoma and neurogenic tumours.
性能
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形式Prediluted
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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存储溶液Preservative: 0.09% Sodium Azide
Constituents: PBS, BSA, pH 7.3 -
Concentration information loading... -
纯度Protein G purified
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Primary antibody说明ab46714 reacts intensely with all carcinomas and metastased derived from the intestinal tract, stomach, pancreas, liver, lung, breast, ovary, thyroid gland, kidney, bladder, prostate. Stratified squamous epithelia usually stain less intensely than adenocarcinoma. The antibody is suitable for differentiation between carcinoma and non-carcinoma. Keratinizing areas of a tumour mass usually remain unstained, as do sarcoma, lymphoma, melanoma and neurogenic tumours.
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克隆单克隆
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克隆编号HEA125
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同种型IgG1
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研究领域
相关产品
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab46714 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | 1/200. | |
| Flow Cyt | Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. | |
| IHC-Fr | Use at an assay dependent concentration. | |
| IHC-P | Use at an assay dependent concentration. |
靶标
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功能May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.
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组织特异性Highly and selectively expressed by undifferentiated rather than differentiated embryonic stem cells (ESC). Levels rapidly diminish as soon as ESC's differentiate (at protein levels). Expressed in almost all epithelial cell membranes but not on mesodermal or neural cell membranes. Found on the surface of adenocarcinoma.
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疾病相关Defects in EPCAM are the cause of diarrhea type 5 (DIAR5) [MIM:613217]. It is an intractable diarrhea of infancy characterized by villous atrophy and absence of inflammation, with intestinal epithelial cell dysplasia manifesting as focal epithelial tufts in the duodenum and jejunum.
Defects in EPCAM are a cause of hereditary non-polyposis colorectal cancer type 8 (HNPCC8) [MIM:613244]. HNPCC is a disease associated with marked increase in cancer susceptibility. It is characterized by a familial predisposition to early-onset colorectal carcinoma (CRC) and extra-colonic tumors of the gastrointestinal, urological and female reproductive tracts. HNPCC is reported to be the most common form of inherited colorectal cancer in the Western world. Clinically, HNPCC is often divided into two subgroups. Type I is characterized by hereditary predisposition to colorectal cancer, a young age of onset, and carcinoma observed in the proximal colon. Type II is characterized by increased risk for cancers in certain tissues such as the uterus, ovary, breast, stomach, small intestine, skin, and larynx in addition to the colon. Diagnosis of classical HNPCC is based on the Amsterdam criteria: 3 or more relatives affected by colorectal cancer, one a first degree relative of the other two; 2 or more generation affected; 1 or more colorectal cancers presenting before 50 years of age; exclusion of hereditary polyposis syndromes. The term 'suspected HNPCC' or 'incomplete HNPCC' can be used to describe families who do not or only partially fulfill the Amsterdam criteria, but in whom a genetic basis for colon cancer is strongly suspected. Note=HNPCC8 results from heterozygous deletion of 3-prime exons of EPCAM and intergenic regions directly upstream of MSH2, resulting in transcriptional read-through and epigenetic silencing of MSH2 in tissues expressing EPCAM. -
序列相似性Belongs to the EPCAM family.
Contains 1 thyroglobulin type-1 domain. -
翻译后修饰Hyperglycosylated in carcinoma tissue as compared with autologous normal epithelia. Glycosylation at Asn-198 is crucial for protein stability.
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细胞定位Lateral cell membrane. Cell junction > tight junction. Co-localizes with CLDN7 at the lateral cell membrane and tight junction.
- Information by UniProt
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数据库链接
- Entrez Gene: 4072 Human
- Omim: 185535 Human
- SwissProt: P16422 Human
- Unigene: 542050 Human
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别名
- 17 1A antibody
- 323/A3 antibody
- Adenocarcinoma associated antigen antibody
see all
Anti-EpCAM antibody [HEA125], prediluted 图像
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![Immunocytochemistry/ Immunofluorescence - Anti-EpCAM antibody [HEA125] (ab46714)](http://www.abcam.cn/ps/products/46/ab46714/Images/ab46714-197421-ab46714gr585861edited.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-EpCAM antibody [HEA125] (ab46714)ICC/IF image of ab46714 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA, 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab46714 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 donkey anti- goat (ab96931) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
实验方案
Anti-EpCAM antibody [HEA125], prediluted (ab46714)参考文献
This product has been referenced in:
- Igarashi S et al. Participation of peribiliary glands in biliary tract pathophysiologies. World J Hepatol 5:425-32 (2013). Read more (PubMed: 24023981) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"