概述

  • 产品名称Anti-EpCAM抗体[E144]
    参阅全部 EpCAM 一抗
  • 描述
    兔单克隆抗体[E144] to EpCAM
  • 特异性This antibody is specific for human EpCAM.
  • 经测试应用适用于: WBmore details
    不适用于: ICC,ICC/IF,IHC-Fr,IHC-P or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues in the C-terminus of Human EpCAM.

  • 阳性对照
    • A431 cell lysate and stomach carcinoma.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab32392 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 39 kDa.
  • 应用说明Is unsuitable for ICC,ICC/IF,IHC-Fr,IHC-P or IP.
  • 靶标

    • 功能May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.
    • 组织特异性Highly and selectively expressed by undifferentiated rather than differentiated embryonic stem cells (ESC). Levels rapidly diminish as soon as ESC's differentiate (at protein levels). Expressed in almost all epithelial cell membranes but not on mesodermal or neural cell membranes. Found on the surface of adenocarcinoma.
    • 疾病相关Defects in EPCAM are the cause of diarrhea type 5 (DIAR5) [MIM:613217]. It is an intractable diarrhea of infancy characterized by villous atrophy and absence of inflammation, with intestinal epithelial cell dysplasia manifesting as focal epithelial tufts in the duodenum and jejunum.
      Defects in EPCAM are a cause of hereditary non-polyposis colorectal cancer type 8 (HNPCC8) [MIM:613244]. HNPCC is a disease associated with marked increase in cancer susceptibility. It is characterized by a familial predisposition to early-onset colorectal carcinoma (CRC) and extra-colonic tumors of the gastrointestinal, urological and female reproductive tracts. HNPCC is reported to be the most common form of inherited colorectal cancer in the Western world. Clinically, HNPCC is often divided into two subgroups. Type I is characterized by hereditary predisposition to colorectal cancer, a young age of onset, and carcinoma observed in the proximal colon. Type II is characterized by increased risk for cancers in certain tissues such as the uterus, ovary, breast, stomach, small intestine, skin, and larynx in addition to the colon. Diagnosis of classical HNPCC is based on the Amsterdam criteria: 3 or more relatives affected by colorectal cancer, one a first degree relative of the other two; 2 or more generation affected; 1 or more colorectal cancers presenting before 50 years of age; exclusion of hereditary polyposis syndromes. The term 'suspected HNPCC' or 'incomplete HNPCC' can be used to describe families who do not or only partially fulfill the Amsterdam criteria, but in whom a genetic basis for colon cancer is strongly suspected. Note=HNPCC8 results from heterozygous deletion of 3-prime exons of EPCAM and intergenic regions directly upstream of MSH2, resulting in transcriptional read-through and epigenetic silencing of MSH2 in tissues expressing EPCAM.
    • 序列相似性Belongs to the EPCAM family.
      Contains 1 thyroglobulin type-1 domain.
    • 翻译后修饰Hyperglycosylated in carcinoma tissue as compared with autologous normal epithelia. Glycosylation at Asn-198 is crucial for protein stability.
    • 细胞定位Lateral cell membrane. Cell junction > tight junction. Co-localizes with CLDN7 at the lateral cell membrane and tight junction.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 17 1A antibody
      • 323/A3 antibody
      • Adenocarcinoma associated antigen antibody
      • Adenocarcinoma-associated antigen antibody
      • Antigen identified by monoclonal antibody AUA1 antibody
      • AUA1 antibody
      • CD326 antibody
      • CD326 antigen antibody
      • Cell surface glycoprotein Trop 1 antibody
      • Cell surface glycoprotein Trop 2 antibody
      • Cell surface glycoprotein Trop-1 antibody
      • CO 17A antibody
      • CO17 1A antibody
      • CO17A antibody
      • DIAR5 antibody
      • EGP 2 antibody
      • EGP antibody
      • EGP2 antibody
      • EGP314 antibody
      • EGP40 antibody
      • Ep CAM antibody
      • Ep-CAM antibody
      • EPCAM antibody
      • EPCAM_HUMAN antibody
      • EpCAM1 antibody
      • Epithelial cell adhesion molecule antibody
      • Epithelial Cell Adhesion Molecule Intracellular Domain (EpCAM-ICD) antibody
      • Epithelial cell surface antigen antibody
      • Epithelial cellular adhesion molecule antibody
      • Epithelial glycoprotein 1 antibody
      • Epithelial glycoprotein 314 antibody
      • Epithelial glycoprotein antibody
      • ESA antibody
      • GA733 1 antibody
      • GA733 2 antibody
      • GA733-2 antibody
      • gastrointestinal tumor-associated antigen 2, 35-KD glycoprotein antibody
      • gp4 antibody
      • hEGP 2 antibody
      • hEGP314 antibody
      • HNPCC8 antibody
      • Human epithelial glycoprotein 2 antibody
      • KS 1/4 antigen antibody
      • KS1/4 antibody
      • KSA antibody
      • Ly74 antibody
      • Lymphocyte antigen 74 antibody
      • M1S 1 antibody
      • M1S2 antibody
      • M4S1 antibody
      • Major gastrointestinal tumor associated protein GA733 2 antibody
      • Major gastrointestinal tumor-associated protein GA733-2 antibody
      • mEGP314 antibody
      • Membrane component chromosome 4 surface marker (35kD glycoprotein) antibody
      • Membrane component, chromosome 4, surface marker 1 antibody
      • Membrane component, chromosome 4, surface marker antibody
      • MIC18 antibody
      • MK 1 antibody
      • Protein 289A antibody
      • TACD1 antibody
      • TACSTD1 antibody
      • TROP1 antibody
      • Tumor associated calcium signal transducer 1 antibody
      • Tumor associated calcium signal transducer 2 precursor antibody
      • Tumor-associated calcium signal transducer 1 antibody
      see all

    Anti-EpCAM antibody [E144] 图像

    • Anti-EpCAM antibody [E144] (ab32392) at 1/2500 dilution + A431 cell lysate

      Predicted band size : 39 kDa

    实验方案

    Anti-EpCAM antibody [E144] (ab32392)参考文献

    This product has been referenced in:
    • Ostenfeld MS  et al. miRNA profiling of circulating EpCAM(+) extracellular vesicles: promising biomarkers of colorectal cancer. J Extracell Vesicles 5:31488 (2016). WB ; Human . Read more (PubMed: 27576678) »
    • Lu WY  et al. Hepatic progenitor cells of biliary origin with liver repopulation capacity. Nat Cell Biol N/A:N/A (2015). WB ; Mouse . Read more (PubMed: 26192438) »

    See all 18 Publications for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Pig Tissue sections (Lung)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate pH6
    Permeabilization No
    Specification Lung
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative 10% normal buffered formalin
    Username

    Abcam user community

    Verified customer

    提交于 Aug 25 2016

    Application Immunohistochemistry (Frozen sections)
    Sample Mouse Tissue sections (stomach)
    Permeabilization No
    Specification stomach
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative Acetone
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    Abcam user community

    Verified customer

    提交于 Aug 22 2016

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (skin tumor)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate pH6
    Permeabilization No
    Specification skin tumor
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative 10% normal buffered formalin
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    Verified customer

    提交于 Aug 15 2016

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (tumor)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate buffer pH6
    Permeabilization No
    Specification tumor
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative Paraformaldehyde
    Username

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    Verified customer

    提交于 Jul 11 2016

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (Ovarian Tumor)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6
    Permeabilization No
    Specification Ovarian Tumor
    Blocking step Peroxidase Blocking Solution + UV block as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: 25°C
    Fixative 58°C - 1h
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    提交于 Jun 02 2016

    Application ELISA
    Sample Human Cell (Cancer cell line)
    Specification Cancer cell line
    Blocking step PBS as blocking agent for 30 minute(s) · Concentration: 15µg/mL · Temperature: 37°C
    Type Direct
    Username

    Abcam user community

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    提交于 Sep 18 2015

    Application Western blot
    Sample Human Cell lysate - whole cell (Colon cancer cell lines)
    Gel Running Conditions Reduced Denaturing (10%)
    Loading amount 20 µg
    Specification Colon cancer cell lines
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
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    提交于 Jun 30 2015

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Specification HeLa
    Permeabilization Yes - 0.5% Triton X100 in PBS
    Fixative Paraformaldehyde
    Username

    Dr. Kirk McManus

    Verified customer

    提交于 Apr 04 2014

    While we do not see other isoforms on SwissProt we do note that it has a number of N glycoslyations which may account for size differences. and that similar banding is seen on Abreviews posted by Abcam customers. We recommend to increase the reducing/...

    Read More
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (Gastrointestinal Tract)
    Specification Gastrointestinal Tract
    Fixative Formalin
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: CC1 Retrieval - Discovery XT of Ventana Medical Systems
    Permeabilization No
    Blocking step OmniMap anti-Rabbit Staining Kit as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: 23°C
    Username

    Abcam user community

    Verified customer

    提交于 Mar 05 2013

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"