Anti-EpCAM抗体[Ber-EP4] (ab7504)

概述

  • 产品名称Anti-EpCAM抗体[Ber-EP4]
    参阅全部 EpCAM 一抗
  • 描述
    小鼠单克隆抗体[Ber-EP4] to EpCAM
  • 特异性Epithelial specific antigen is cell surface glycoprotein and is broadly distributed in epithelial cells and displays a highly conserved expression in carcinomas. Epithelial specific antigen has been know to play an important role as tumor-cell marker in lymph nodes from patients with esophageal carcinoma. Epithelial specific antigen can be used to distinguish between basal cell and basosquamous carcinomas and squamous cell carcinoma of the skin.
  • 经测试应用适用于: IHC-FoFr, IHC-P, IHC-Frmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    BALB/C mice were immunized with human breast carcinoma cell line, MCF-7.

  • 阳性对照
    • Kidney tissue sections.

性能

应用

Our Abpromise guarantee covers the use of ab7504 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-FoFr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.

靶标

  • 功能May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.
  • 组织特异性Highly and selectively expressed by undifferentiated rather than differentiated embryonic stem cells (ESC). Levels rapidly diminish as soon as ESC's differentiate (at protein levels). Expressed in almost all epithelial cell membranes but not on mesodermal or neural cell membranes. Found on the surface of adenocarcinoma.
  • 疾病相关Defects in EPCAM are the cause of diarrhea type 5 (DIAR5) [MIM:613217]. It is an intractable diarrhea of infancy characterized by villous atrophy and absence of inflammation, with intestinal epithelial cell dysplasia manifesting as focal epithelial tufts in the duodenum and jejunum.
    Defects in EPCAM are a cause of hereditary non-polyposis colorectal cancer type 8 (HNPCC8) [MIM:613244]. HNPCC is a disease associated with marked increase in cancer susceptibility. It is characterized by a familial predisposition to early-onset colorectal carcinoma (CRC) and extra-colonic tumors of the gastrointestinal, urological and female reproductive tracts. HNPCC is reported to be the most common form of inherited colorectal cancer in the Western world. Clinically, HNPCC is often divided into two subgroups. Type I is characterized by hereditary predisposition to colorectal cancer, a young age of onset, and carcinoma observed in the proximal colon. Type II is characterized by increased risk for cancers in certain tissues such as the uterus, ovary, breast, stomach, small intestine, skin, and larynx in addition to the colon. Diagnosis of classical HNPCC is based on the Amsterdam criteria: 3 or more relatives affected by colorectal cancer, one a first degree relative of the other two; 2 or more generation affected; 1 or more colorectal cancers presenting before 50 years of age; exclusion of hereditary polyposis syndromes. The term 'suspected HNPCC' or 'incomplete HNPCC' can be used to describe families who do not or only partially fulfill the Amsterdam criteria, but in whom a genetic basis for colon cancer is strongly suspected. Note=HNPCC8 results from heterozygous deletion of 3-prime exons of EPCAM and intergenic regions directly upstream of MSH2, resulting in transcriptional read-through and epigenetic silencing of MSH2 in tissues expressing EPCAM.
  • 序列相似性Belongs to the EPCAM family.
    Contains 1 thyroglobulin type-1 domain.
  • 翻译后修饰Hyperglycosylated in carcinoma tissue as compared with autologous normal epithelia. Glycosylation at Asn-198 is crucial for protein stability.
  • 细胞定位Lateral cell membrane. Cell junction > tight junction. Co-localizes with CLDN7 at the lateral cell membrane and tight junction.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 17 1A antibody
    • 323/A3 antibody
    • Adenocarcinoma associated antigen antibody
    • Adenocarcinoma-associated antigen antibody
    • Antigen identified by monoclonal antibody AUA1 antibody
    • AUA1 antibody
    • CD326 antibody
    • CD326 antigen antibody
    • Cell surface glycoprotein Trop 1 antibody
    • Cell surface glycoprotein Trop 2 antibody
    • Cell surface glycoprotein Trop-1 antibody
    • CO 17A antibody
    • CO17 1A antibody
    • CO17A antibody
    • DIAR5 antibody
    • EGP 2 antibody
    • EGP antibody
    • EGP2 antibody
    • EGP314 antibody
    • EGP40 antibody
    • Ep CAM antibody
    • Ep-CAM antibody
    • EPCAM antibody
    • EPCAM_HUMAN antibody
    • EpCAM1 antibody
    • Epithelial cell adhesion molecule antibody
    • Epithelial Cell Adhesion Molecule Intracellular Domain (EpCAM-ICD) antibody
    • Epithelial cell surface antigen antibody
    • Epithelial cellular adhesion molecule antibody
    • Epithelial glycoprotein 1 antibody
    • Epithelial glycoprotein 314 antibody
    • Epithelial glycoprotein antibody
    • ESA antibody
    • GA733 1 antibody
    • GA733 2 antibody
    • GA733-2 antibody
    • gastrointestinal tumor-associated antigen 2, 35-KD glycoprotein antibody
    • gp4 antibody
    • hEGP 2 antibody
    • hEGP314 antibody
    • HNPCC8 antibody
    • Human epithelial glycoprotein 2 antibody
    • KS 1/4 antigen antibody
    • KS1/4 antibody
    • KSA antibody
    • Ly74 antibody
    • Lymphocyte antigen 74 antibody
    • M1S 1 antibody
    • M1S2 antibody
    • M4S1 antibody
    • Major gastrointestinal tumor associated protein GA733 2 antibody
    • Major gastrointestinal tumor-associated protein GA733-2 antibody
    • mEGP314 antibody
    • Membrane component chromosome 4 surface marker (35kD glycoprotein) antibody
    • Membrane component, chromosome 4, surface marker 1 antibody
    • Membrane component, chromosome 4, surface marker antibody
    • MIC18 antibody
    • MK 1 antibody
    • Protein 289A antibody
    • TACD1 antibody
    • TACSTD1 antibody
    • TROP1 antibody
    • Tumor associated calcium signal transducer 1 antibody
    • Tumor associated calcium signal transducer 2 precursor antibody
    • Tumor-associated calcium signal transducer 1 antibody
    see all

Anti-EpCAM antibody [Ber-EP4] 图像

  • ab7504 staining EpCAM in Human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% serum for 1 hour at 20°C; antigen retrieval was by heat mediation (microwave) in a commercial retrieval buffer. Samples were incubated with primary antibody (1/250 in 1% serum) for 12 hours at 4°C. A Biotin-conjugated Horse anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • ab7504 staining EpCAM in formalin-fixed, paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry. Staining was detected using DAB.

Anti-EpCAM antibody [Ber-EP4] (ab7504)参考文献

This product has been referenced in:
  • Kobayashi H  et al. Expression of the GA733 gene family and its relationship to prognosis in pulmonary adenocarcinoma. Virchows Arch 457:69-76 (2010). IHC-P ; Human . Read more (PubMed: 20473768) »
  • Pan MR  et al. Tubocapsanolide A inhibits transforming growth factor-beta-activating kinase 1 to suppress NF-kappaB-induced CCR7. J Biol Chem 284:2746-54 (2009). IHC-P ; Human . Read more (PubMed: 19049961) »

See all 2 Publications for this product

Product Wall

Application Flow Cytometry
Sample Human Cell (Breast cancer cell (MCF7))
Permeabilization No
Gating Strategy Standard
Specification Breast cancer cell (MCF7)
Preparation Cell harvesting/tissue preparation method: Adherent. MCF7 cell line was cultured in DMEM medium, 10% FBS. Volume used was 15 mL for 75 cm2 flask.
Sample buffer: DMEM + 10% FBS
Username

Miss. Soledad Carinelli

Verified customer

提交于 May 20 2015


TAG72 antibody, ab16838, Lot GR99749-1: 66.6 ug/ml


Epcam antibody (Ber-EP4), ab7504, Lot GR103717-1: 47.3 ug/ml

Thank you for contacting us.

Unfortunately, this antibody has not been purified and therefore, its concentration has not been determined. However, we estimate that the antibody concentration in this tissue culture supernatant is 1-3 mg/ml. <...

Read More

Thank you for your inquiry.

For ab758, the immunogen that was used was cytoskeletal extract of human hepatocellular carcinoma cell line, Hep3B. We have not validated this antibody in pig. But the human sequence is 85% homologous with the pig ...

Read More

Thank you for contacting us.

I was not able to find a purified, concentrated formulation of the Ber-EP4 clone in our catalogue, and we have not had it in the past.

Please do not hesitate to contact us if you need any more advice o...

Read More

Thank you for contacting us. As shown on the datasheet of the anti-EpCAM ab71916, www.abcam.com/ab71916, SW480 whole cell lysate is a recommended positive control. As explained on the phone, to our knowledge ab7504 has been tested in Western Blot...

Read More

Thank you for contacting us in regards to Anti-EpCAM antibody [Ber-EP4] (ab7504) and Anti-EpCAM antibody [HEA125] (ab46714). As discussed over the phone yesterday, we do not currently have any information regarding these antibodies having been used in ...

Read More

Thank you for contacting us. THE A431, HCT116 and MCF etc express this target so lysates of these can be used as positive control. For more information about the tissue that expresses this target please check Human Protein Atlas. As discussed; ...

Read More

Thank you for contacting us. As discussed on phone the antibody in question can be used with ab7504. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for contacting us.  We have not tested ab7504 for use on mouse samples.  The antibody was raised against the full length protein, which is 81% homologous with mouse EpCAM, so there is a chance that it may cross-react with mouse samples.  Unfo...

Read More

1-10 of 16 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"