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Tissue, cells or virus corresponding to Human EpCAM. Raised against carcinoma cell line of human origin.
Dilute according to the particular application being used. In general, 0.05M borate pH 8.0 containing 0.15M sodium chloride, 0.02% sodium azide, is a good dilutent to use with most antibodies. Avoid diluting the entire contents of the vial at once since the diluted solution may have reduced stability.
Fluorescein (FITC) conjugated at a ratio of 6 moles of Fluorescein to 1 mole of antibody. Maximum excitation of FITC occurs at wavelength 492 nm and maximum emission occurs at wavelength 520 nm.
Our Abpromise guarantee covers the use of ab8666 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use a concentration of 5 - 10 µg/ml. PubMed: 17417652|
|IHC-P||Use a concentration of 5 - 10 µg/ml. PubMed: 20421921|
|WB||Use a concentration of 1 - 5 µg/ml.
This product was not quality controlled in Flow Cytometry applications. However, it may be applicable for Flow Cytometry at a concentration of 1-2 µg in 0.1 ml containing 10^6 cells.
|IP||Use at an assay dependent concentration.|
|ICC/IF||Use a concentration of 5 - 10 µg/ml. See Abreview.|
Histogram plot of prostate tumour cells DU145 (green) over isotype control (red) stained with EpCam following resection.
This picture was kindly supplied as part of the review submitted by Phil Kearney.