Anti-EPC1抗体(ab112043)
Key features and details
- Rabbit polyclonal to EPC1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-EPC1抗体 -
描述
兔多克隆抗体to EPC1 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Rabbit, Horse, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant -
免疫原
Synthetic peptide, corresponding to a region between amino acids 325-375 of Human EPC1 (NP_079485.1).
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阳性对照
- HeLa whole cell lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
7 to 8 -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab112043于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 93 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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说明 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 93 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
靶标
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功能
Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when directly recruited to sites of DNA damage. -
序列相似性
Belongs to the enhancer of polycomb family. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 450393 Chimpanzee
- Entrez Gene: 80314 Human
- Entrez Gene: 13831 Mouse
- Entrez Gene: 100362678 Rat
- Omim: 610999 Human
- SwissProt: Q9H2F5 Human
- SwissProt: Q8C9X6 Mouse
- Unigene: 167805 Human
see all -
别名
- DKFZp781P2312 antibody
- Enhancer of polycomb 1 antibody
- Enhancer of polycomb homolog 1 (Drosophila) antibody
see all
图片
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All lanes : Anti-EPC1 antibody (ab112043) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 93 kDa
Exposure time: 3 minutes -
Detection of EPC1 by Western Blot of Immunprecipitate.
ab112043 at 1µg/ml staining EPC1 in HeLa whole cell lysate immunoprecipitated using ab112043 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab112043 被引用在 1 文献中.
- Wang Y et al. Epigenetic factor EPC1 is a master regulator of DNA damage response by interacting with E2F1 to silence death and activate metastasis-related gene signatures. Nucleic Acids Res 44:117-33 (2016). ChIP . PubMed: 26350215