概述

  • 产品名称Anti-EPB42抗体
    参阅全部 EPB42 一抗
  • 描述
    小鼠单克隆抗体to EPB42
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment: KMPEKAEQYQ PLTASVSLQN SLDAPMEDCV ISILGRGLIH RERSYRFRSV WPENTMCAKF QFTPTHVGLQ RLTVEVDCNM FQNLTNYKSV TVVAPELSA, corresponding to amino acids 623-722 of Human EPB42 with a 26kDa enzyme tag (NP_000110).

  • 阳性对照
    • EPB42 transfected 293T lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆单克隆
  • 同种型IgG2a
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab76910 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent dilution.
WB 1/500 - 1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).

靶标

  • 功能Probably plays an important role in the regulation of erythrocyte shape and mechanical properties.
  • 疾病相关Defects in EPB42 are the cause of spherocytosis type 5 (SPH5) [MIM:612690]; also known as hereditary spherocytosis type 5 (HS5). Spherocytosis is a hematologic disorder leading to chronic hemolytic anemia and characterized by numerous abnormally shaped erythrocytes which are generally spheroidal. Absence of band 4.2 associated with spur or target erythrocytes has also been reported.
  • 序列相似性Belongs to the transglutaminase superfamily. Transglutaminase family.
  • 翻译后修饰Both cAMP-dependent kinase (CAPK) and another kinase present in the red-blood cells seem to be able to phosphorylate EPB42.
  • 细胞定位Cell membrane. Cytoplasm > cytoskeleton. Cytoplasmic surface of erythrocyte membranes.
  • Information by UniProt
  • 数据库链接
  • 别名
    • EPB42 antibody
    • EPB42_HUMAN antibody
    • Erythrocyte membrane protein band 4.2 antibody
    • Erythrocyte protein 4.2 antibody
    • Erythrocyte surface protein band 4.2 antibody
    • MGC116735 antibody
    • MGC116737 antibody
    • P4.2 antibody
    • PA antibody
    • SPH5 antibody
    see all

Anti-EPB42 antibody 图像

  • Anti-EPB42 antibody (ab76910) at 1/500 dilution + Recombinant fragment of human EPB42 at 0.1 µg

    Secondary
    Goat anti-Mouse IgG (H&L)-HRP

    Predicted band size : 70 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-EPB42 antibody (ab76910) at 1/500 dilution

    Lane 1 : EPB42 transfected 293T lysate
    Lane 2 : Non-transfected 293T lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 70 kDa
    Observed band size : 70 kDa

Anti-EPB42 antibody (ab76910)参考文献

ab76910 has not yet been referenced specifically in any publications.

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