RabMAb

Anti-Epac1抗体[EPR1672] (ab109415)

概述

  • 产品名称Anti-Epac1抗体[EPR1672]
    参阅全部 Epac1 一抗
  • 描述
    兔单克隆抗体[EPR1672] to Epac1
  • 经测试应用适用于: WB, IP, IHC-P, ICCmore details
    不适用于: Flow Cyt
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues in the C-terminus of Human Epac1.

  • 阳性对照
    • HeLa, HepG2, Human heart and HUVEC lysates; Human kidney tissue
  • 常规说明

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 纯度Tissue culture supernatant
  • 克隆单克隆
  • 克隆编号EPR1672
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab109415 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 104 kDa.
IP 1/10 - 1/100.
IHC-P 1/50 - 1/100. Antigen retrieval is recommended.
ICC 1/50 - 1/100.
  • 应用说明Is unsuitable for Flow Cyt.
  • 靶标

    • 相关性The activation of RaP1 by cAMP is independent of PKA and is mediated by recently discovered family of guanine nucleotide exchange factors (GEFs) called cAMP-GEFs or Epacs. The Epac signaling therefore represents a novel mechanism for cAMP signaling with in the cAMP cascade. There are 2 members of the Epac family, Epac1 and Epac 2. Both proteins are multidomain proteins containing an autoinhibitory cAMP-binding domain that inhibits the catalytic region and a DEP domain (dishevelled, Egl-10 and pleckstrin homology domain) targeting the membrane anchors. EPAC2 has an additional cAMP-binding site in its N-terminus that binds cAMP with low affinity. EPAC1 mRNA is broadly expressed, with particularly high levels occurring in the thyroid, ovary, kidney and certain brain regions, whereas expression of EPAC2 mRNA appears to be restricted to the brain and adrenal glands. Epac 1 and Epac 2 also interact with light chain 2 (LC2) or MAP1A that serves as a scaffolding structure to stabilize the signal transduction complex. The Epac 1-selective antibodies were generated against unique antigenic sequences form near N-terminus and between RasGEFN and Ras GEF domains. The antibodies to Epac 1are affinity purified over immobilized antigen based chromatography.
    • 细胞定位Endomembrane system
    • 数据库链接
    • 别名
      • bcm910 antibody
      • CAMP GEFI antibody
      • cAMP regulated guanine nucleotide exchange factor I antibody
      • CAMPGEFI antibody
      • CGEF 1 antibody
      • CGEF1 antibody
      • EPA1 antibody
      • Epac 1 antibody
      • EPAC antibody
      • EPAC1 antibody
      • Exchange factor directly activated by cAMP 1 antibody
      • Exchange protein directly activated by cAMP 1 antibody
      • MGC21410 antibody
      • RAP guanine nucleotide exchange factor antibody
      • Rap guanine nucleotide exchange factor (GEF) 3 antibody
      • RAP guanine nucleotide exchange factor 3 antibody
      • Rap1 guanine nucleotide exchange factor directly activated by cAMP antibody
      • RAPGEF3 antibody
      see all

    Anti-Epac1 antibody [EPR1672] 图像

    • All lanes : Anti-Epac1 antibody [EPR1672] (ab109415) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : Human heart lysate
      Lane 4 : HUVEC cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 104 kDa
    • Immunohistochemical analysis of Epac1 in paraffin-embedded Human kidney tissue using ab109415 at 1/50 dilution.

    Anti-Epac1 antibody [EPR1672] (ab109415)参考文献

    ab109415 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HUVEC)
    Gel Running Conditions Reduced Denaturing
    Loading amount 35 µg
    Specification HUVEC
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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    提交于 Jan 21 2016

    Thank you for your inquiry.

    Unfortunately, we are not able to release the immunogen sequence for this particular antibody as it is a proprietary information. This antibody is fully guaranteed for human, mouse and rat, species. In case you ar...

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    Thank you for contacting us.
    We can confirm that the immunogen for catalogue number ab124162 is within the first 180aa.
    This antibody is guaranteed to be used in WB, other application hasn't been tested yet. if you are considering using this ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"