概述

  • 产品名称Anti-Eotaxin抗体
    参阅全部 Eotaxin 一抗
  • 描述
    兔多克隆抗体to Eotaxin
  • 经测试应用适用于: ICC/IF, IHC-R, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide, corresponding to amino acids 83-97 of Human Eotaxin.

  • 阳性对照
    • The recombinant protein can be used as positive control.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab8018 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
IHC-R Use at an assay dependent concentration.
WB Use a concentration of 0.5 - 1 µg/ml. Can be blocked with Eotaxin peptide (ab8127). Monomer and homodimer are detected at approximate 9 and 18 kDa.

靶标

  • 功能In response to the presence of allergens, this protein directly promotes the accumulation of eosinophils, a prominent feature of allergic inflammatory reactions. Binds to CCR3.
  • 序列相似性Belongs to the intercrine beta (chemokine CC) family.
  • 翻译后修饰O-linked glycan consists of a Gal-GalNAc disaccharide which is mofified with up to 2 sialic acid residues.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C-C motif chemokine 11 antibody
    • CCL 11 antibody
    • CCL11 antibody
    • CCL11_HUMAN antibody
    • chemokine (C-C motif) ligand 11 antibody
    • Chemokine C C motif ligand 11 antibody
    • Chemokine CC Motif Ligand 11 antibody
    • Chemokine ligand 11 antibody
    • Eosinophil chemotactic protein antibody
    • Eotaxin antibody
    • eotaxin-1 antibody
    • MGC22554 antibody
    • SCYA 11 antibody
    • SCYA11 antibody
    • Small inducible cytokine A11 antibody
    • Small inducible cytokine subfamily A (Cys Cys) member 11 antibody
    • small inducible cytokine subfamily A (Cys-Cys), member 11 (eotaxin) antibody
    • Small inducible cytokine subfamily A member 11 antibody
    • Small-inducible cytokine A11 antibody
    see all

Anti-Eotaxin antibody 图像

  • All lanes : Anti-Eotaxin antibody (ab8018) at 1 µg/ml

    Lane 1 : Hela cell lysate containing 10ng full length recombinant eotaxin
    Lane 2 : Hela cell lysate containing 2.5ng full length recombinant eotaxin
    Lane 3 : Hela cell lysate containing 1ng full length recombinant eotaxin
  • ab8018 at 5µg/ml staining Eotaxin in 293 cells by ICC/IF

  • ab8018 staining Eotaxin in Human nasal polyp and tonsil tissue sections by Immunohistochemistry (resin-embedded sections). The tissue was fixed in acetone and inhibitors and embedded into GMA resin. Samples were incubated with primary antibody (1/250 in Tris buffered saline) for 18 hours at 4°C. A Biotin-conjugated Pig anti-rabbit polyclonal (1/1200) was used as the secondary antibody.

    See Abreview

  • Immunofluorescence of Eotaxin in 293 cells using ab8018 at 10 ug/ml.

Anti-Eotaxin antibody (ab8018)参考文献

This product has been referenced in:
  • Mo FM  et al. Interferon {gamma}-Inducible Protein-10 (IP-10) and Eotaxin as Biomarkers in Age-Related Macular Degeneration. Invest Ophthalmol Vis Sci 51:4226-36 (2010). Read more (PubMed: 20220052) »

See 1 Publication for this product

Product Wall

Thank you very much for your interest in our Eotaxin antibodies.

I would recommend using ab133604 in your experiments because it will be covered under our Abpromise guarantee for use in IHC-P on human samples. If you would like to test ab801...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Resin sections)
Sample Human Tissue sections (Nasal polyp, tonsil, bronchial biopsies)
Specification Nasal polyp, tonsil, bronchial biopsies
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Verified customer

提交于 Sep 28 2010

To date, both antibodies have only been tested in Western Blotting

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"