The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 - 2 µg/ml.
Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Goat polyclonal to Eotaxin 3 (Biotin) (ab83505). This antigen affinity purified antibody, in conjunction with ab83505 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant human Eotaxin 3.
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 11 kDa.
Use a concentration of 5 - 7 µg/ml. To yield one-half maximal inhibition [ND50] of the biological activity of Human Eotaxin 3 (100 ng/ml)
Chemotactic for eosinophils and basophils. Binds to CCR3.
Ubiquitously expressed at low levels in various tissues including heart and ovary.
Belongs to the intercrine beta (chemokine CC) family.
Mateo T et al. Angiotensin II-induced mononuclear leukocyte interactions with arteriolar and venular endothelium are mediated by the release of different CC chemokines. J Immunol176:5577-86 (2006).
Read more (PubMed: 16622027) »
Cuvelier SL & Patel KD Shear-dependent eosinophil transmigration on interleukin 4-stimulated endothelial cells: a role for endothelium-associated eotaxin-3. J Exp Med194:1699-709 (2001).
Read more (PubMed: 11748272) »