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Our Abpromise guarantee covers the use of ab84016 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use a concentration of 0.25 - 1 µg/ml. Allows the detection of at least 0.2 - 0.4 ng/well of recombinant mouse Eotaxin 2.|
|Sandwich ELISA||Use a concentration of 0.25 - 1 µg/ml. Can be paired for Sandwich ELISA with Goat polyclonal to Eotaxin 2 (Biotin) (ab84015). Allows the detection of at least 0.2 - 0.4 ng/well of recombinant mouse Eotaxin 2. Can be used as Capture antibody when paired with ab84015.|
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 13 kDa. The detection limit for recombinant mouse Eotaxin 2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.|
|Neutralising||Use a concentration of 7 - 8 µg/ml. To yield one-half maximal inhibition [ND50] of the biological activity of mouse Eotaxin 2 (100 ng/ml).|
ab84016 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"