概述

  • 产品名称Anti-ENY2抗体
    参阅全部 ENY2 一抗
  • 描述
    兔多克隆抗体to ENY2
  • 经测试应用适用于: ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    antigen sequence: AINQKLMETG ERERLKELLR AKLIECGWKD QLKAHCKEVI KEKGLEHVTV DDLVAEITPK GRALVPDSVK KELLQRIRT, corresponding to amino acids 15-93 of Human ENY2.

  • 阳性对照
    • Human rectum tissue.

性能

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应用

Our Abpromise guarantee covers the use of ab121836 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Component of the transcription regulatory histone acetylation (HAT) complex SAGA, a multiprotein complex that activates transcription by remodeling chromatin and mediating histone acetylation and deubiquitination. Within the SAGA complex, participates to a subcomplex that specifically deubiquitinates both histones H2A and H2B. The SAGA complex is recruited to specific gene promoters by activators such as MYC, where it is required for transcription. Required for nuclear receptor-mediated transactivation. May also participate in mRNA export and accurate chromatin positioning in the nucleus by tethering genes to the nuclear periphery.
  • 序列相似性Belongs to the ENY2 family.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 1810057B09Rik antibody
    • 6720481I12 antibody
    • DC6 antibody
    • e(y)2 antibody
    • Enhancer of yellow 2 transcription factor homolog antibody
    • eny2 antibody
    • ENY2_HUMAN antibody
    • Ey2 antibody
    see all

Anti-ENY2 antibody 图像

  • Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli and mitochondria. Recommended concentration of ab121836 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121836 at 1/40 dilution staining ENY2 in Paraffin-embedded Human Rectum tissue by Immunohistochemistry. Note: strong positivity in glandular cells.

Anti-ENY2 antibody (ab121836)参考文献

ab121836 has not yet been referenced specifically in any publications.

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