概述

  • 产品名称Anti-ENTPD5抗体[EPR3784]
    参阅全部 ENTPD5 一抗
  • 描述
    兔单克隆抗体[EPR3784] to ENTPD5
  • 经测试应用适用于: WB, IP, IHC-Pmore details
    不适用于: Flow Cyt or ICC
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (internal sequence)

  • 阳性对照
    • Fetal liver cell lysate; Human kidney tissue
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab108603 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 47 kDa (predicted molecular weight: 48 kDa).Can be blocked with ENTPD5 peptide (ab190069).
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Antigen retrieval is recommended.
  • 应用说明Is unsuitable for Flow Cyt or ICC.
  • 靶标

    • 功能Uridine diphosphatase (UDPase) that promotes protein N-glycosylation and ATP level regulation. UDP hydrolysis promotes protein N-glycosylation and folding in the endoplasmic reticulum, as well as elevated ATP consumption in the cytosol via an ATP hydrolysis cycle. Together with CMPK1 and AK1, constitutes an ATP hydrolysis cycle that converts ATP to AMP and results in a compensatory increase in aerobic glycolysis. Also hydrolyzes GDP and IDP but not any other nucleoside di-, mono- or triphosphates, nor thiamine pyrophosphate. Plays a key role in the AKT1-PTEN signaling pathway by promoting glycolysis in proliferating cells in response to phosphoinositide 3-kinase (PI3K) signaling.
    • 组织特异性Expressed in adult liver, kidney, prostate, testis and colon. Much weaker expression in other tissues.
    • 通路Protein modification; protein glycosylation.
    • 序列相似性Belongs to the GDA1/CD39 NTPase family.
    • 翻译后修饰N-glycosylated; high-mannose type.
    • 细胞定位Endoplasmic reticulum lumen.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CD39 like 4 antibody
      • AI196558 antibody
      • AI987697 antibody
      • CD39 antigen like 4 antibody
      • CD39 antigen-like 4 antibody
      • CD39 like 4 antibody
      • CD39L4 antibody
      • Ectonucleoside triphosphate diphosphohydrolase 5 antibody
      • ENTP5_HUMAN antibody
      • Entpd5 antibody
      • ER UDPase antibody
      • ER-UDPase antibody
      • GDPase ENTPD5 antibody
      • Guanosine-diphosphatase ENTPD5 antibody
      • MGC163357 antibody
      • MGC163359 antibody
      • mNTPase antibody
      • NTPDase 5 antibody
      • Nucleoside diphosphatase antibody
      • PCPH antibody
      • Pcph proto oncogene protein antibody
      • Proto oncogene CPH antibody
      • UDPase ENTPD5 antibody
      • Uridine-diphosphatase ENTPD5 antibody
      see all

    Anti-ENTPD5 antibody [EPR3784] 图像

    • Anti-ENTPD5 antibody [EPR3784] (ab108603) at 1/1000 dilution + fetal liver cell lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 48 kDa
      Observed band size : 47 kDa (why is the actual band size different from the predicted?)
    • ab108603, at 1/100 dilution, staining ENTPD5 in paraffin-embedded Human kidney tissue by Immunohistochemistry.

    Anti-ENTPD5 antibody [EPR3784] (ab108603)参考文献

    This product has been referenced in:
    • Walsh J  et al. Identification and quantification of the basal and inducible Nrf2-dependent proteomes in mouse liver: biochemical, pharmacological and toxicological implications. J Proteomics 108:171-87 (2014). WB ; Mouse . Read more (PubMed: 24859727) »
    • Curry NL  et al. Pten-null tumors cohabiting the same lung display differential AKT activation and sensitivity to dietary restriction. Cancer Discov 3:908-21 (2013). WB, IHC-P ; Mouse . Read more (PubMed: 23719831) »

    See all 2 Publications for this product

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Sample Mouse Cell (Spermatozoa)
    Specification Spermatozoa
    Permeabilization Yes - Triton X 0.25%
    Fixative Paraformaldehyde
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    提交于 May 30 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"