Anti-Enterokinase抗体(ab79974)

概述

  • 产品名称Anti-Enterokinase抗体
    参阅全部 Enterokinase 一抗
  • 描述
    兔多克隆抗体to Enterokinase
  • 经测试应用适用于: IHC-Pmore details
  • 种属反应性

    预测可用于: Horse, Cow, Pig
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 950 to the C-terminus of Human Enterokinase.

  • 阳性对照
    • In IHC, this antibody gave a positive signal in a human duodenum formalin fixed paraffin embedded tissue section.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab79974 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Responsible for initiating activation of pancreatic proteolytic proenzymes (trypsin, chymotrypsin and carboxypeptidase A). It catalyzes the conversion of trypsinogen to trypsin which in turn activates other proenzymes including chymotrypsinogen, procarboxypeptidases, and proelastases.
  • 组织特异性Intestinal brush border.
  • 疾病相关Defects in TMPRSS15 are a cause of enterokinase deficiency (ENTKD) [MIM:226200]; a life-threatening intestinal malabsorption disorder characterized by diarrhea and failure to thrive.
  • 序列相似性Belongs to the peptidase S1 family.
    Contains 2 CUB domains.
    Contains 2 LDL-receptor class A domains.
    Contains 1 MAM domain.
    Contains 1 peptidase S1 domain.
    Contains 1 SEA domain.
    Contains 1 SRCR domain.
  • 翻译后修饰The chains are derived from a single precursor that is cleaved by a trypsin-like protease.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • PRSS 7 antibody
    • PRSS7 antibody
    • Enterokinase antibody
    • Enterokinase catalytic subunit antibody
    • Enteropeptidase antibody
    • Enteropeptidase catalytic light chain antibody
    • Enteropeptidase non catalytic heavy chain antibody
    • ENTK antibody
    • ENTK_HUMAN antibody
    • PROENTEROKINASE antibody
    • protease, serine, 7 (enterokinase) antibody
    • PRSS7 antibody
    • Serine protease 7 antibody
    • TMPRSS15 antibody
    • Transmembrane protease serine 15 antibody
    see all

Anti-Enterokinase antibody 图像

  • IHC image of Enterokinase staining in human duodenum formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab79974, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Enterokinase antibody (ab79974)参考文献

ab79974 has not yet been referenced specifically in any publications.

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