Anti-Entactin抗体(ab77179)

概述

  • 产品名称Anti-Entactin抗体
    参阅全部 Entactin 一抗
  • 描述
    小鼠单克隆抗体to Entactin
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Cow, Dog, Chimpanzee, Macaque Monkey
  • 免疫原

    GST-tagged recombinant fragment: EGLQYPFAVT SYGKNLYFTD WKMNSVVALD LAISKETDAF QPHKQTRLYG ITTALSQCPQ GHNYCSVNNG GCTHLCLATP GSRTCRCPDN TLGVDCIEQK, corresponding to C terminal amino acids 1148-1247 of Human Entactin (NP_002499

  • 阳性对照
    • Entactin transfected 293T cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab77179 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 136 kDa.
ELISA Use at an assay dependent dilution.

靶标

Anti-Entactin antibody 图像

  • Anti-Entactin antibody (ab77179) at 5 µg/ml + immunogen (GST-tagged 100 aa recombinant fragment) at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L) HRP-conjugated at 1/5000 dilution

    Predicted band size : 136 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-Entactin antibody (ab77179) at 5 µg/ml

    Lane 1 : Entactin transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L) HRP-conjugated at 1/2500 dilution

    Predicted band size : 136 kDa
    Observed band size : 136 kDa

Anti-Entactin antibody (ab77179)参考文献

ab77179 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your message and for confirming these details. I am very pleased to hear the customer would like to accept our offer and test these antibodies in hamster. ab41684 Discount code: #### ab38975 Discount code: #### ab6314 ...

Read More

Thank you for your message which has been forwarded to me as Karin is currently away from the office. I have reviewed these three antibodies with respect to discount codes: ab77179 I am sorry I am also unable to check the alignment of the immun...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"