概述

  • 产品名称Anti-ENT1抗体
    参阅全部 ENT1 一抗
  • 描述
    兔多克隆抗体to ENT1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Cynomolgus Monkey, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human ENT1.

    (Peptide available as ab48606.)

  • 阳性对照
    • This antibody gave a positive signal in Human Heart Tissue Lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab48607 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 2 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 50 kDa).Can be blocked with Human ENT1 peptide (ab48606).

靶标

  • 功能Mediates both influx and efflux of nucleosides across the membrane (equilibrative transporter). It is sensitive (ES) to low concentrations of the inhibitor nitrobenzylmercaptopurine riboside (NBMPR) and is sodium-independent. It has a higher affinity for adenosine. Inhibited by dipyridamole and dilazep (anticancer chemotherapeutics drugs).
  • 组织特异性Expressed in heart, brain, mammary gland, erythrocytes and placenta, and also in fetal liver and spleen.
  • 序列相似性Belongs to the SLC29A transporter family.
  • 翻译后修饰Glycosylated.
  • 细胞定位Basolateral cell membrane. Apical cell membrane. Predominantly localized in the basolateral membrane in polarised MDCK cells.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Equilibrative NBMPR-sensitive nucleoside transporter antibody
    • equilibrative nitrobenzylmercaptopurine riboside (NBMPR)-sensitive nucleoside transporter antibody
    • Equilibrative nitrobenzylmercaptopurine riboside-sensitive nucleoside transporter antibody
    • Equilibrative nucleoside transporter 1 antibody
    • es-type antibody
    • MGC1465 antibody
    • MGC3778 antibody
    • Nucleoside transporter antibody
    • Nucleoside transporter, es-type antibody
    • OTTHUMP00000016506 antibody
    • OTTHUMP00000016507 antibody
    • OTTHUMP00000016508 antibody
    • OTTHUMP00000016509 antibody
    • OTTHUMP00000016510 antibody
    • OTTHUMP00000016511 antibody
    • OTTHUMP00000016512 antibody
    • S29A1_HUMAN antibody
    • Slc29a1 antibody
    • solute carrier family 29 (equilibrative nucleoside transporter), member 1 antibody
    • solute carrier family 29 (nucleoside transporters), member 1 antibody
    • Solute carrier family 29 member 1 antibody
    see all

Anti-ENT1 antibody 图像

  • All lanes : Anti-ENT1 antibody (ab48607) at 2 µg/ml

    Lane 1 : Human heart tissue lysate - total protein (ab29431)
    Lane 2 : Human brain tissue lysate - total protein (ab29466)
    Lane 3 : Human heart tissue lysate - total protein (ab29431) with Human ENT1 peptide (ab48606) at 2 µg/ml
    Lane 4 : Human brain tissue lysate - total protein (ab29466) with Human ENT1 peptide (ab48606) at 2 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 50 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 36 kDa,78 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes

Anti-ENT1 antibody (ab48607)参考文献

This product has been referenced in:
  • Osgood CL  et al. (18)F-FLT Positron Emission Tomography (PET) is a Pharmacodynamic Marker for EWS-FLI1 Activity and Ewing Sarcoma. Sci Rep 6:33926 (2016). Read more (PubMed: 27671553) »
  • Kerr M  et al. Deoxycytidine kinase expression underpins response to gemcitabine in bladder cancer. Clin Cancer Res 20:5435-45 (2014). Read more (PubMed: 25224279) »

See all 5 Publications for this product

Product Wall

Thank you for contacting us. The Anti-ENT1 antibody (ab48607) is raised against residues 250-350 of human ENT1 (SwissProt reference Q99808). As yet this antibody has not been tested for its activity against the mouse protein and as the region used ...

Read More
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Brain)
Specification Brain
Fixative Paraformaldehyde
Permeabilization Yes - Triton X-100
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jun 01 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HEK293 #U251 #MCF-7)
Loading amount 30 µg
Specification HEK293 #U251 #MCF-7
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Mr. Antibody Solutions

Verified customer

提交于 Sep 17 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"