概述

  • 产品名称Anti-ENPP6抗体
    参阅全部 ENPP6 一抗
  • 描述
    兔多克隆抗体to ENPP6
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 360-409 (LMDMRGIFLA FGPDFKSNFR AAPIRSVDVY NVMCNVVGIT PLPNNGSWSR) of Human ENPP6 (NP_699174).

  • 阳性对照
    • 721_B cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab87587 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 50 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 相关性ENPP6 is a choline-specific glycerophosphodiester phosphodiesterase, which hydrolyzes the classical substrate for phospholipase C, p-nitrophenyl phosphorylcholine, while it does not hydrolyze the classical nucleotide phosphodiesterase substrate, p-nitrophenyl thymidine 5 prime-monophosphate. It hydrolyzes lysophosphatidylcholine (LPC) to form monoacylglycerol and phosphorylcholine but not lysophosphatidic acid, showing it has a lysophospholipase C activity. ENPP6 has a preference for LPC with short (12:0 and 14:0) or polyunsaturated (18:2 and 20:4) fatty acids. ENPP6 also hydrolyzes glycerophosphorylcholine and sphingosylphosphorylcholine efficiently. ENPP6 is predominantly expressed in kidney and brain.
  • 细胞定位Cell membrane; Single-pass type I membrane protein. Secreted. Note: A minor secreted form also exists.
  • 数据库链接
  • 别名
    • B830047L21Rik antibody
    • E-NPP 6 antibody
    • Ectonucleotide pyrophosphatase/phosphodiesterase 6 antibody
    • ectonucleotide pyrophosphatase/phosphodiesterase family member 6 antibody
    • ENPP 6 antibody
    • NPP 6 antibody
    • NPP6 antibody
    see all

Anti-ENPP6 antibody 图像

  • Anti-ENPP6 antibody (ab87587) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 50 kDa
    Observed band size : 50 kDa
    Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.

Anti-ENPP6 antibody (ab87587)参考文献

ab87587 has not yet been referenced specifically in any publications.

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